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. 1994 Jun;60(6):2017–2022. doi: 10.1128/aem.60.6.2017-2022.1994

Effects of Temperature, pH, and NaCl on Growth and Pectinolytic Activity of Pseudomonas marginalis

J M Membré 1,*, P M Burlot 1
PMCID: PMC201596  PMID: 16349288

Abstract

The interaction of temperature (4, 10, 18, and 30°C), pH (6, 7, and 8), and NaCl (0, 2.5, and 5%) and their effects on specific growth rate, lag phase, and pectinolytic enzymes of Pseudomonas marginalis were evaluated. Response surface methodology was adapted to describe the response of growth parameters to environmental changes. To obtain good conditions of storage, the combined action of salt and temperature is necessary. At 4°C with an NaCl concentration of 5% and a pH of 7, the lag time was 8 days and no growth was observed at 4°C with 5% NaCl and a pH of 6. In the absence of salt, P. marginalis could grow regardless of temperature and pH. Pectate lyase and pectin lyase were produced by P. marginalis, while pectin methyl esterase activity was not observed in our culture conditions. The enzyme production depended on temperature, pH, and salt concentration but also on the age of the culture. Pectinolytic enzymes were abundantly excreted during the stationary phase, and even at 4°C, after 2 weeks of storage, enzyme activities in supernatant culture were sufficient to damage vegetables. Both bacterial growth and enzymatic production have to be taken into account in order to estimate correctly the shelf life of vegetables.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brocklehurst T. F., Zaman-Wong C. M., Lund B. M. A note on the microbiology of retail packs of prepared salad vegetables. J Appl Bacteriol. 1987 Nov;63(5):409–415. doi: 10.1111/j.1365-2672.1987.tb04862.x. [DOI] [PubMed] [Google Scholar]
  2. Davey K. R. A predictive model for combined temperature and water activity on microbial growth during the growth phase. J Appl Bacteriol. 1989 Nov;67(5):483–488. doi: 10.1111/j.1365-2672.1989.tb02519.x. [DOI] [PubMed] [Google Scholar]
  3. Gügi B., Orange N., Hellio F., Burini J. F., Guillou C., Leriche F., Guespin-Michel J. F. Effect of growth temperature on several exported enzyme activities in the psychrotrophic bacterium Pseudomonas fluorescens. J Bacteriol. 1991 Jun;173(12):3814–3820. doi: 10.1128/jb.173.12.3814-3820.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Kiers L., King J. O. Increased intracranial pressure following bilateral neck dissection and radiotherapy. Aust N Z J Surg. 1991 Jun;61(6):459–461. doi: 10.1111/j.1445-2197.1991.tb00263.x. [DOI] [PubMed] [Google Scholar]
  5. Liao C. H. Analysis of pectate lyases produced by soft rot bacteria associated with spoilage of vegetables. Appl Environ Microbiol. 1989 Jul;55(7):1677–1683. doi: 10.1128/aem.55.7.1677-1683.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Nasuno S., Starr M. P. Pectic enzymes of pseudomonas marginalis. Phytopathology. 1966 Dec;56(12):1414–1415. [PubMed] [Google Scholar]
  7. Nikaidou N., Kamio Y., Izaki K. Molecular cloning and nucleotide sequence of a pectin lyase gene from Pseudomonas marginalis N6301. Biochem Biophys Res Commun. 1992 Jan 15;182(1):14–19. doi: 10.1016/s0006-291x(05)80105-6. [DOI] [PubMed] [Google Scholar]
  8. Schoolfield R. M., Sharpe P. J., Magnuson C. E. Non-linear regression of biological temperature-dependent rate models based on absolute reaction-rate theory. J Theor Biol. 1981 Feb 21;88(4):719–731. doi: 10.1016/0022-5193(81)90246-0. [DOI] [PubMed] [Google Scholar]
  9. Zwietering M. H., Jongenburger I., Rombouts F. M., van 't Riet K. Modeling of the bacterial growth curve. Appl Environ Microbiol. 1990 Jun;56(6):1875–1881. doi: 10.1128/aem.56.6.1875-1881.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]

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