Skip to main content
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1994 Jun;60(6):2158–2163. doi: 10.1128/aem.60.6.2158-2163.1994

Detection, purification, and partial characterization of plantaricin C, a bacteriocin produced by a Lactobacillus plantarum strain of dairy origin.

B González 1, P Arca 1, B Mayo 1, J E Suárez 1
PMCID: PMC201617  PMID: 8080262

Abstract

A bacteriocin produced by Lactobacillus plantarum LL441 was selected from the inhibitory products of 75 mesophilic lactobacilli because of its potency and broad spectrum. It is a peptide of 3.5 kDa whose amino-terminal sequence is NH2-K-K-T-K-K-N-X-S-G-D-I-. It is bactericidal and, in some cases, bacteriolytic. The peptide, called plantaricin C, retained its activity after boiling, storage, and treatment at different pHs.

Full text

PDF

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barefoot S. F., Klaenhammer T. R. Purification and characterization of the Lactobacillus acidophilus bacteriocin lactacin B. Antimicrob Agents Chemother. 1984 Sep;26(3):328–334. doi: 10.1128/aac.26.3.328. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Biswas S. R., Ray P., Johnson M. C., Ray B. Influence of Growth Conditions on the Production of a Bacteriocin, Pediocin AcH, by Pediococcus acidilactici H. Appl Environ Microbiol. 1991 Apr;57(4):1265–1267. doi: 10.1128/aem.57.4.1265-1267.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Davey G. P., Richardson B. C. Purification and Some Properties of Diplococcin from Streptococcus cremoris 346. Appl Environ Microbiol. 1981 Jan;41(1):84–89. doi: 10.1128/aem.41.1.84-89.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Edman P., Begg G. A protein sequenator. Eur J Biochem. 1967 Mar;1(1):80–91. doi: 10.1007/978-3-662-25813-2_14. [DOI] [PubMed] [Google Scholar]
  5. Fleming H. P., Etchells J. L., Costilow R. N. Microbial inhibition by an isolate of pediococcus from cucumber brines. Appl Microbiol. 1975 Dec;30(6):1040–1042. doi: 10.1128/am.30.6.1040-1042.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gálvez A., Maqueda M., Valdivia E., Quesada A., Montoya E. Characterization and partial purification of a broad spectrum antibiotic AS-48 produced by Streptococcus faecalis. Can J Microbiol. 1986 Oct;32(10):765–771. doi: 10.1139/m86-141. [DOI] [PubMed] [Google Scholar]
  7. Hastings J. W., Sailer M., Johnson K., Roy K. L., Vederas J. C., Stiles M. E. Characterization of leucocin A-UAL 187 and cloning of the bacteriocin gene from Leuconostoc gelidum. J Bacteriol. 1991 Dec;173(23):7491–7500. doi: 10.1128/jb.173.23.7491-7500.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Jiménez-Díaz R., Rios-Sánchez R. M., Desmazeaud M., Ruiz-Barba J. L., Piard J. C. Plantaricins S and T, Two New Bacteriocins Produced by Lactobacillus plantarum LPCO10 Isolated from a Green Olive Fermentation. Appl Environ Microbiol. 1993 May;59(5):1416–1424. doi: 10.1128/aem.59.5.1416-1424.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Joerger M. C., Klaenhammer T. R. Characterization and purification of helveticin J and evidence for a chromosomally determined bacteriocin produced by Lactobacillus helveticus 481. J Bacteriol. 1986 Aug;167(2):439–446. doi: 10.1128/jb.167.2.439-446.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kellner R., Jung G., Hörner T., Zähner H., Schnell N., Entian K. D., Götz F. Gallidermin: a new lanthionine-containing polypeptide antibiotic. Eur J Biochem. 1988 Oct 15;177(1):53–59. doi: 10.1111/j.1432-1033.1988.tb14344.x. [DOI] [PubMed] [Google Scholar]
  11. Klaenhammer T. R. Bacteriocins of lactic acid bacteria. Biochimie. 1988 Mar;70(3):337–349. doi: 10.1016/0300-9084(88)90206-4. [DOI] [PubMed] [Google Scholar]
  12. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  13. Lindgren S. E., Dobrogosz W. J. Antagonistic activities of lactic acid bacteria in food and feed fermentations. FEMS Microbiol Rev. 1990 Sep;7(1-2):149–163. doi: 10.1111/j.1574-6968.1990.tb04885.x. [DOI] [PubMed] [Google Scholar]
  14. Schillinger U., Lücke F. K. Antibacterial activity of Lactobacillus sake isolated from meat. Appl Environ Microbiol. 1989 Aug;55(8):1901–1906. doi: 10.1128/aem.55.8.1901-1906.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Schved F., Lalazar A., Henis Y., Juven B. J. Purification, partial characterization and plasmid-linkage of pediocin SJ-1, a bacteriocin produced by Pediococcus acidilactici. J Appl Bacteriol. 1993 Jan;74(1):67–77. doi: 10.1111/j.1365-2672.1993.tb02998.x. [DOI] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES