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. 1994 Jun;60(6):2191–2195. doi: 10.1128/aem.60.6.2191-2195.1994

Purification and partial amino acid sequence of curvaticin FS47, a heat-stable bacteriocin produced by Lactobacillus curvatus FS47.

K I Garver 1, P M Muriana 1
PMCID: PMC201625  PMID: 8031103

Abstract

Curvaticin FS47, a bacteriocin produced by Lactobacillus curvatus FS47, is inhibitory to Listeria monocytogenes, as well as Lactobacillus, Pediococcus, Enterococcus, and Bacillus spp. The bacteriocin was purified by 40% ammonium sulfate precipitation, solid-phase extraction, and reversed-phase high-pressure liquid chromatography. Purified curvaticin FS47 was determined to be 4.07 kDa by mass spectrometry and was partially sequenced. Thirty-one N-terminal amino acids were identified; the curvaticin FS47 protein sequence did not show homology to the pediocin-like group of bacteriocins.

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Selected References

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  1. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. Basic local alignment search tool. J Mol Biol. 1990 Oct 5;215(3):403–410. doi: 10.1016/S0022-2836(05)80360-2. [DOI] [PubMed] [Google Scholar]
  2. Armon R., Arella M., Payment P. A highly efficient second-step concentration technique for bacteriophages and enteric viruses using ammonium sulfate and Tween 80. Can J Microbiol. 1988 May;34(5):651–655. doi: 10.1139/m88-107. [DOI] [PubMed] [Google Scholar]
  3. Barefoot S. F., Klaenhammer T. R. Detection and activity of lactacin B, a bacteriocin produced by Lactobacillus acidophilus. Appl Environ Microbiol. 1983 Jun;45(6):1808–1815. doi: 10.1128/aem.45.6.1808-1815.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Christensen B., Fink J., Merrifield R. B., Mauzerall D. Channel-forming properties of cecropins and related model compounds incorporated into planar lipid membranes. Proc Natl Acad Sci U S A. 1988 Jul;85(14):5072–5076. doi: 10.1073/pnas.85.14.5072. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Furth A. J., Bolton H., Potter J., Priddle J. D. Separating detergent from proteins. Methods Enzymol. 1984;104:318–328. doi: 10.1016/s0076-6879(84)04098-2. [DOI] [PubMed] [Google Scholar]
  6. Garver K. I., Muriana P. M. Detection, identification and characterization of bacteriocin-producing lactic acid bacteria from retail food products. Int J Food Microbiol. 1993 Sep;19(4):241–258. doi: 10.1016/0168-1605(93)90017-b. [DOI] [PubMed] [Google Scholar]
  7. Hastings J. W., Sailer M., Johnson K., Roy K. L., Vederas J. C., Stiles M. E. Characterization of leucocin A-UAL 187 and cloning of the bacteriocin gene from Leuconostoc gelidum. J Bacteriol. 1991 Dec;173(23):7491–7500. doi: 10.1128/jb.173.23.7491-7500.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Henderson J. T., Chopko A. L., van Wassenaar P. D. Purification and primary structure of pediocin PA-1 produced by Pediococcus acidilactici PAC-1.0. Arch Biochem Biophys. 1992 May 15;295(1):5–12. doi: 10.1016/0003-9861(92)90480-k. [DOI] [PubMed] [Google Scholar]
  9. Héchard Y., Dérijard B., Letellier F., Cenatiempo Y. Characterization and purification of mesentericin Y105, an anti-Listeria bacteriocin from Leuconostoc mesenteroides. J Gen Microbiol. 1992 Dec;138(12):2725–2731. doi: 10.1099/00221287-138-12-2725. [DOI] [PubMed] [Google Scholar]
  10. Jung D. S., Bodyfelt F. W., Daeschel M. A. Influence of fat and emulsifiers on the efficacy of nisin in inhibiting Listeria monocytogenes in fluid milk. J Dairy Sci. 1992 Feb;75(2):387–393. doi: 10.3168/jds.S0022-0302(92)77773-X. [DOI] [PubMed] [Google Scholar]
  11. Klaenhammer T. R. Genetics of bacteriocins produced by lactic acid bacteria. FEMS Microbiol Rev. 1993 Sep;12(1-3):39–85. doi: 10.1111/j.1574-6976.1993.tb00012.x. [DOI] [PubMed] [Google Scholar]
  12. Lewus C. B., Kaiser A., Montville T. J. Inhibition of food-borne bacterial pathogens by bacteriocins from lactic acid bacteria isolated from meat. Appl Environ Microbiol. 1991 Jun;57(6):1683–1688. doi: 10.1128/aem.57.6.1683-1688.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Muriana P. M., Klaenhammer T. R. Cloning, phenotypic expression, and DNA sequence of the gene for lactacin F, an antimicrobial peptide produced by Lactobacillus spp. J Bacteriol. 1991 Mar;173(5):1779–1788. doi: 10.1128/jb.173.5.1779-1788.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Muriana P. M., Klaenhammer T. R. Purification and partial characterization of lactacin F, a bacteriocin produced by Lactobacillus acidophilus 11088. Appl Environ Microbiol. 1991 Jan;57(1):114–121. doi: 10.1128/aem.57.1.114-121.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Mørtvedt C. I., Nissen-Meyer J., Sletten K., Nes I. F. Purification and amino acid sequence of lactocin S, a bacteriocin produced by Lactobacillus sake L45. Appl Environ Microbiol. 1991 Jun;57(6):1829–1834. doi: 10.1128/aem.57.6.1829-1834.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Nieto Lozano J. C., Meyer J. N., Sletten K., Peláz C., Nes I. F. Purification and amino acid sequence of a bacteriocin produced by Pediococcus acidilactici. J Gen Microbiol. 1992 Sep;138(9):1985–1990. doi: 10.1099/00221287-138-9-1985. [DOI] [PubMed] [Google Scholar]
  17. Nissen-Meyer J., Holo H., Håvarstein L. S., Sletten K., Nes I. F. A novel lactococcal bacteriocin whose activity depends on the complementary action of two peptides. J Bacteriol. 1992 Sep;174(17):5686–5692. doi: 10.1128/jb.174.17.5686-5692.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Nissen-Meyer J., Larsen A. G., Sletten K., Daeschel M., Nes I. F. Purification and characterization of plantaricin A, a Lactobacillus plantarum bacteriocin whose activity depends on the action of two peptides. J Gen Microbiol. 1993 Sep;139(9):1973–1978. doi: 10.1099/00221287-139-9-1973. [DOI] [PubMed] [Google Scholar]
  19. Reese E. T., Maguire A. Surfactants as stimulants of enzyme production by microorganisms. Appl Microbiol. 1969 Feb;17(2):242–245. doi: 10.1128/am.17.2.242-245.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Schillinger U., Kaya M., Lücke F. K. Behaviour of Listeria monocytogenes in meat and its control by a bacteriocin-producing strain of Lactobacillus sake. J Appl Bacteriol. 1991 Jun;70(6):473–478. doi: 10.1111/j.1365-2672.1991.tb02743.x. [DOI] [PubMed] [Google Scholar]
  21. Schillinger U., Lücke F. K. Antibacterial activity of Lactobacillus sake isolated from meat. Appl Environ Microbiol. 1989 Aug;55(8):1901–1906. doi: 10.1128/aem.55.8.1901-1906.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Schved F., Lalazar A., Henis Y., Juven B. J. Purification, partial characterization and plasmid-linkage of pediocin SJ-1, a bacteriocin produced by Pediococcus acidilactici. J Appl Bacteriol. 1993 Jan;74(1):67–77. doi: 10.1111/j.1365-2672.1993.tb02998.x. [DOI] [PubMed] [Google Scholar]
  23. Schägger H., von Jagow G. Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal Biochem. 1987 Nov 1;166(2):368–379. doi: 10.1016/0003-2697(87)90587-2. [DOI] [PubMed] [Google Scholar]
  24. Sobrino O. J., Rodríguez J. M., Moreira W. L., Cintas L. M., Fernández M. F., Sanz B., Hernández P. E. Sakacin M, a bacteriocin-like substance from Lactobacillus sake 148. Int J Food Microbiol. 1992 Jul;16(3):215–225. doi: 10.1016/0168-1605(92)90082-e. [DOI] [PubMed] [Google Scholar]
  25. Sobrino O. J., Rodríguez J. M., Moreira W. L., Fernández M. F., Sanz B., Hernández P. E. Antibacterial activity of Lactobacillus sake isolated from dry fermented sausages. Int J Food Microbiol. 1991 May;13(1):1–10. doi: 10.1016/0168-1605(91)90130-h. [DOI] [PubMed] [Google Scholar]
  26. Stoffels G., Nissen-Meyer J., Gudmundsdottir A., Sletten K., Holo H., Nes I. F. Purification and characterization of a new bacteriocin isolated from a Carnobacterium sp. Appl Environ Microbiol. 1992 May;58(5):1417–1422. doi: 10.1128/aem.58.5.1417-1422.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Umesaki Y., Kawai Y., Mutai M. Effect of Tween 80 on glucosyltransferase production in Streptococcus mutans. Appl Environ Microbiol. 1977 Aug;34(2):115–119. doi: 10.1128/aem.34.2.115-119.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. van Belkum M. J., Hayema B. J., Jeeninga R. E., Kok J., Venema G. Organization and nucleotide sequences of two lactococcal bacteriocin operons. Appl Environ Microbiol. 1991 Feb;57(2):492–498. doi: 10.1128/aem.57.2.492-498.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. van Laack R. L., Schillinger U., Holzapfel W. H. Characterization and partial purification of a bacteriocin produced by Leuconostoc carnosum LA44A. Int J Food Microbiol. 1992 Jul;16(3):183–195. doi: 10.1016/0168-1605(92)90079-i. [DOI] [PubMed] [Google Scholar]

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