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. 1994 Jul;60(7):2339–2342. doi: 10.1128/aem.60.7.2339-2342.1994

Carbon Metabolism Enzymes of Rhizobium tropici Cultures and Bacteroids

Vassily I Romanov 1,2,*, Ismael Hernández-Lucas 2, Esperanza Martínez-Romero 2
PMCID: PMC201652  PMID: 16349319

Abstract

We determined the activities of selected enzymes involved in carbon metabolism in free-living cells of Rhizobium tropici CFN299 grown in minimal medium with different carbon sources and in bacteroids of the same strain. The set of enzymatic activities in sucrose-grown cells suggests that the pentose phosphate pathway, with the participation of the Entner-Doudoroff pathway, is probably the primary route for sugar catabolism. In glutamate- and malate-grown cells, high activities of the gluconeogenic enzymes (phosphoenolpyruvate carboxykinase, fructose-6-phosphate aldolase, and fructose bisphosphatase) were detected. In bacteroids, isolated in Percoll gradients, the levels of activity for many of the enzymes measured were similar to those of malate-grown cells, except that higher activities of glucokinase, glucose-6-phosphate dehydrogenase, and NAD-dependent phosphogluconate dehydrogenase were detected. Phosphoglucomutase and UDP glucose pyrophosphorylase showed high and constant levels under all growth conditions and in bacteroids.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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