Skip to main content
PMC home page
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1994 Jul;60(7):2653–2656. doi: 10.1128/aem.60.7.2653-2656.1994

Expression of Heterogenous Arsenic Resistance Genes in the Obligately Autotrophic Biomining Bacterium Thiobacillus ferrooxidans

Ji-Bin Peng 1,*, Wang-Ming Yan 1, Xue-Zhen Bao 1
PMCID: PMC201699  PMID: 16349341

Abstract

Two arsenic-resistant plasmids were constructed and introduced into Thiobacillus ferrooxidans strains by conjugation. The plasmids with the replicon of wide-host-range plasmid RSF1010 were stable in T. ferrooxidans. The arsenic resistance genes originating from the heterotroph were expressed in this obligately autotrophic bacterium, but the promoter derived from T. ferrooxidans showed no special function in its original host.

Full text

PDF
2653

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barros M. E., Rawlings D. E., Woods D. R. Cloning and expression of the Thiobacillus ferrooxidans glutamine synthetase gene in Escherichia coli. J Bacteriol. 1985 Dec;164(3):1386–1389. doi: 10.1128/jb.164.3.1386-1389.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Barros M. E., Rawlings D. E., Woods D. R. Production and Regeneration of Thiobacillus ferrooxidans Spheroplasts. Appl Environ Microbiol. 1985 Sep;50(3):721–723. doi: 10.1128/aem.50.3.721-723.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brierley C. L. Bacterial leaching. CRC Crit Rev Microbiol. 1978;6(3):207–26I. doi: 10.3109/10408417809090623. [DOI] [PubMed] [Google Scholar]
  4. Datta N., Hedges R. W., Shaw E. J., Sykes R. B., Richmond M. H. Properties of an R factor from Pseudomonas aeruginosa. J Bacteriol. 1971 Dec;108(3):1244–1249. doi: 10.1128/jb.108.3.1244-1249.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Davison J., Heusterspreute M., Chevalier N., Ha-Thi V., Brunel F. Vectors with restriction site banks. V. pJRD215, a wide-host-range cosmid vector with multiple cloning sites. Gene. 1987;51(2-3):275–280. doi: 10.1016/0378-1119(87)90316-7. [DOI] [PubMed] [Google Scholar]
  6. Hoffman L. E., Hendrix J. L. Inhibition of Thiobacillus ferrooxidans by soluble silver. Biotechnol Bioeng. 1976 Aug;18(8):1161–1165. doi: 10.1002/bit.260180811. [DOI] [PubMed] [Google Scholar]
  7. Kusano T., Sugawara K., Inoue C., Takeshima T., Numata M., Shiratori T. Electrotransformation of Thiobacillus ferrooxidans with plasmids containing a mer determinant. J Bacteriol. 1992 Oct;174(20):6617–6623. doi: 10.1128/jb.174.20.6617-6623.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kusano T., Takeshima T., Sugawara K., Inoue C., Shiratori T., Yano T., Fukumori Y., Yamanaka T. Molecular cloning of the gene encoding Thiobacillus ferrooxidans Fe(II) oxidase. High homology of the gene product with HiPIP. J Biol Chem. 1992 Jun 5;267(16):11242–11247. [PubMed] [Google Scholar]
  9. Martin P. A., Dugan P. R., Tuovinen O. H. Plasmid DNA in acidophilic, chemolithotrophic thiobacilli. Can J Microbiol. 1981 Aug;27(8):850–853. doi: 10.1139/m81-133. [DOI] [PubMed] [Google Scholar]
  10. Mobley H. L., Chen C. M., Silver S., Rosen B. P. Cloning and expression of R-factor mediated arsenate resistance in Escherichia coli. Mol Gen Genet. 1983;191(3):421–426. doi: 10.1007/BF00425757. [DOI] [PubMed] [Google Scholar]
  11. Murray N. E., Brammar W. J., Murray K. Lambdoid phages that simplify the recovery of in vitro recombinants. Mol Gen Genet. 1977 Jan 7;150(1):53–61. doi: 10.1007/BF02425325. [DOI] [PubMed] [Google Scholar]
  12. Peng J. B., Yan W. M., Bao X. Z. Plasmid and transposon transfer to Thiobacillus ferrooxidans. J Bacteriol. 1994 May;176(10):2892–2897. doi: 10.1128/jb.176.10.2892-2897.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. SILVERMAN M. P., LUNDGREN D. G. Studies on the chemoautotrophic iron bacterium Ferrobacillus ferrooxidans. I. An improved medium and a harvesting procedure for securing high cell yields. J Bacteriol. 1959 May;77(5):642–647. doi: 10.1128/jb.77.5.642-647.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Shiratori T., Inoue C., Sugawara K., Kusano T., Kitagawa Y. Cloning and expression of Thiobacillus ferrooxidans mercury ion resistance genes in Escherichia coli. J Bacteriol. 1989 Jun;171(6):3458–3464. doi: 10.1128/jb.171.6.3458-3464.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Stüber D., Bujard H. Organization of transcriptional signals in plasmids pBR322 and pACYC184. Proc Natl Acad Sci U S A. 1981 Jan;78(1):167–171. doi: 10.1073/pnas.78.1.167. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Tuovinen O. H., Niemelä S. I., Gyllenberg H. G. Tolerance of Thiobacillus ferrooxidans to some metals. Antonie Van Leeuwenhoek. 1971;37(4):489–496. doi: 10.1007/BF02218519. [DOI] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES