Abstract
From contaminated industrial sludge, two stable multistrain microbial enrichments (consortia) that were capable of rapidly utilizing chlorobenzene and o-dichlorobenzene, respectively, were obtained. These consortia were characterized as to their species composition, tolerance range, and activity maxima in order to establish and maintain the required operational parameters during their use in biofilters for the removal of chlorobenzene contaminants from air. The consortia were immobilized on a porous perlite support packed into filter columns. Metered airstreams containing the contaminant vapors were partially humidified and passed through these columns. The vapor concentrations prior to and after biofiltration were measured by gas chromatography. Liquid was circulated concurrently with the air, and the device was operated in the trickling air biofilter mode. The experimental arrangement allowed the independent variation of liquid flow, airflow, and solvent vapor concentrations. Bench-scale trickling air biofilters removed monochlorobenzene, o-dichlorobenzene, and their mixtures at rates of up to 300 g of solvent vapor h(-1) m(-3) filter volume. High liquid recirculation rates and automated pH control were critical for stable filtration performance. When the accumulating NaCl was periodically diluted, the trickling air biofilters continued to remove chlorobenzenes for several months with no loss of activity. The demonstrated high performance and stability of the described trickling air biofilters favor their use in industrial-scale air pollution control.
Full text
PDF





Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
- Haigler B. E., Nishino S. F., Spain J. C. Degradation of 1,2-dichlorobenzene by a Pseudomonas sp. Appl Environ Microbiol. 1988 Feb;54(2):294–301. doi: 10.1128/aem.54.2.294-301.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kröckel L., Focht D. D. Construction of chlorobenzene-utilizing recombinants by progenitive manifestation of a rare event. Appl Environ Microbiol. 1987 Oct;53(10):2470–2475. doi: 10.1128/aem.53.10.2470-2475.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nishino S. F., Spain J. C., Belcher L. A., Litchfield C. D. Chlorobenzene degradation by bacteria isolated from contaminated groundwater. Appl Environ Microbiol. 1992 May;58(5):1719–1726. doi: 10.1128/aem.58.5.1719-1726.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pettigrew C. A., Haigler B. E., Spain J. C. Simultaneous biodegradation of chlorobenzene and toluene by a Pseudomonas strain. Appl Environ Microbiol. 1991 Jan;57(1):157–162. doi: 10.1128/aem.57.1.157-162.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Reineke W., Knackmuss H. J. Microbial metabolism of haloaromatics: isolation and properties of a chlorobenzene-degrading bacterium. Appl Environ Microbiol. 1984 Feb;47(2):395–402. doi: 10.1128/aem.47.2.395-402.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Spain J. C., Nishino S. F. Degradation of 1,4-dichlorobenzene by a Pseudomonas sp. Appl Environ Microbiol. 1987 May;53(5):1010–1019. doi: 10.1128/aem.53.5.1010-1019.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- de Bont J. A., Vorage M. J., Hartmans S., van den Tweel W. J. Microbial degradation of 1,3-dichlorobenzene. Appl Environ Microbiol. 1986 Oct;52(4):677–680. doi: 10.1128/aem.52.4.677-680.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
