Abstract
A 1.8-kb chromosomal DNA fragment of the mycoplasmalike organism (MLO) associated with apple proliferation was sequenced. Three putative open reading frames were observed on this fragment. The protein encoded by open reading frame 2 shows significant homologies with bacterial nitroreductases. From the nucleotide sequence four primer pairs for PCR were chosen to specifically amplify DNA from MLOs associated with European diseases of fruit trees. Primer pairs specific for (i) Malus-affecting MLOs, (ii) Malus- and Prunus-affecting MLOs, and (iii) Malus-, Prunus-, and Pyrus-affecting MLOs were obtained. Restriction enzyme analysis of the amplification products revealed restriction fragment length polymorphisms between Malus-, Prunus, and Pyrus-affecting MLOs as well as between different isolates of the apple proliferation MLO. No amplification with either primer pair could be obtained with DNA from 12 different MLOs experimentally maintained in periwinkle.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bryant C., Hubbard L., McElroy W. D. Cloning, nucleotide sequence, and expression of the nitroreductase gene from Enterobacter cloacae. J Biol Chem. 1991 Mar 5;266(7):4126–4130. [PubMed] [Google Scholar]
- Karow M., Georgopoulos C. Sequencing, mutational analysis, and transcriptional regulation of the Escherichia coli htrB gene. Mol Microbiol. 1991 Sep;5(9):2285–2292. doi: 10.1111/j.1365-2958.1991.tb02159.x. [DOI] [PubMed] [Google Scholar]
- Kirkpatrick B. C., Stenger D. C., Morris T. J., Purcell A. H. Cloning and Detection of DNA from a Nonculturable Plant Pathogenic Mycoplasma-like Organism. Science. 1987 Oct 9;238(4824):197–200. doi: 10.1126/science.238.4824.197. [DOI] [PubMed] [Google Scholar]
- Kuske C. R., Kirkpatrick B. C. Phylogenetic relationships between the western aster yellows mycoplasmalike organism and other prokaryotes established by 16S rRNA gene sequence. Int J Syst Bacteriol. 1992 Apr;42(2):226–233. doi: 10.1099/00207713-42-2-226. [DOI] [PubMed] [Google Scholar]
- Lee I. M., Gundersen D. E., Davis R. E., Chiykowski L. N. Identification and analysis of a genomic strain cluster of mycoplasmalike organisms associated with Canadian peach (eastern) X disease, western X disease, and clover yellow edge. J Bacteriol. 1992 Oct;174(20):6694–6698. doi: 10.1128/jb.174.20.6694-6698.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lim P. O., Sears B. B. 16S rRNA sequence indicates that plant-pathogenic mycoplasmalike organisms are evolutionarily distinct from animal mycoplasmas. J Bacteriol. 1989 Nov;171(11):5901–5906. doi: 10.1128/jb.171.11.5901-5906.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lim P. O., Sears B. B. DNA sequence of the ribosomal protein genes rp12 and rps19 from a plant-pathogenic mycoplasma-like organism. FEMS Microbiol Lett. 1991 Nov 1;68(1):71–73. doi: 10.1016/0378-1097(91)90397-s. [DOI] [PubMed] [Google Scholar]
- Lim P. O., Sears B. B. Evolutionary relationships of a plant-pathogenic mycoplasmalike organism and Acholeplasma laidlawii deduced from two ribosomal protein gene sequences. J Bacteriol. 1992 Apr;174(8):2606–2611. doi: 10.1128/jb.174.8.2606-2611.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schaff D., Lee I. M., Davis R. E. Sensitive detection and identification of mycoplasma-like organisms in plants by polymerase chain reactions. Biochem Biophys Res Commun. 1992 Aug 14;186(3):1503–1509. doi: 10.1016/s0006-291x(05)81576-1. [DOI] [PubMed] [Google Scholar]
- Watanabe M., Ishidate M., Jr, Nohmi T. Nucleotide sequence of Salmonella typhimurium nitroreductase gene. Nucleic Acids Res. 1990 Feb 25;18(4):1059–1059. doi: 10.1093/nar/18.4.1059. [DOI] [PMC free article] [PubMed] [Google Scholar]