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. 1994 Sep;60(9):3368–3374. doi: 10.1128/aem.60.9.3368-3374.1994

Cometabolic degradation of trichloroethylene by Pseudomonas cepacia G4 in a chemostat with toluene as the primary substrate.

A S Landa 1, E M Sipkema 1, J Weijma 1, A A Beenackers 1, J Dolfing 1, D B Janssen 1
PMCID: PMC201811  PMID: 7524444

Abstract

Pseudomonas cepacia G4 is capable of cometabolic degradation of trichloroethylene (TCE) if the organism is grown on certain aromatic compounds. To obtain more insight into the kinetics of TCE degradation and the effect of TCE transformation products, we have investigated the simultaneous conversion of toluene and TCE in steady-state continuous culture. The organism was grown in a chemostat with toluene as the carbon and energy source at a range of volumetric TCE loading rates, up to 330 mumol/liter/h. The specific TCE degradation activity of the cells and the volumetric activity increased, but the efficiency of TCE conversion dropped when the TCE loading was elevated from 7 to 330 mumol/liter/h. At TCE loading rates of up to 145 mumol/liter/h, the specific toluene conversion rate and the molar growth yield of the cells were not affected by the presence of TCE. The response of the system to varying TCE loading rates was accurately described by a mathematical model based on Michaelis-Menten kinetics and competitive inhibition. A high load of 3,400 mumol of TCE per liter per h for 12 h caused inhibition of toluene and TCE conversion, but reduction of the TCE load to the original nontoxic level resulted in complete recovery of the system within 2 days. These results show that P. cepacia can stably and continuously degrade toluene and TCE simultaneously in a single-reactor system without biomass retention and that the organism is more resistant to high concentrations and shock loadings of TCE than Methylosinus trichosporium OB3b.

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Selected References

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  1. Alvarez-Cohen L., McCarty P. L. Effects of toxicity, aeration, and reductant supply on trichloroethylene transformation by a mixed methanotrophic culture. Appl Environ Microbiol. 1991 Jan;57(1):228–235. doi: 10.1128/aem.57.1.228-235.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Ensley B. D. Biochemical diversity of trichloroethylene metabolism. Annu Rev Microbiol. 1991;45:283–299. doi: 10.1146/annurev.mi.45.100191.001435. [DOI] [PubMed] [Google Scholar]
  3. Ensley B. D., Kurisko P. R. A gas lift bioreactor for removal of contaminants from the vapor phase. Appl Environ Microbiol. 1994 Jan;60(1):285–290. doi: 10.1128/aem.60.1.285-290.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Ewers J., Freier-Schröder D., Knackmuss H. J. Selection of trichloroethene (TCE) degrading bacteria that resist inactivation by TCE. Arch Microbiol. 1990;154(4):410–413. doi: 10.1007/BF00276540. [DOI] [PubMed] [Google Scholar]
  5. Folsom B. R., Chapman P. J. Performance characterization of a model bioreactor for the biodegradation of trichloroethylene by Pseudomonas cepacia G4. Appl Environ Microbiol. 1991 Jun;57(6):1602–1608. doi: 10.1128/aem.57.6.1602-1608.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Folsom B. R., Chapman P. J., Pritchard P. H. Phenol and trichloroethylene degradation by Pseudomonas cepacia G4: kinetics and interactions between substrates. Appl Environ Microbiol. 1990 May;56(5):1279–1285. doi: 10.1128/aem.56.5.1279-1285.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Henry S. M., Grbić-Galić D. Influence of endogenous and exogenous electron donors and trichloroethylene oxidation toxicity on trichloroethylene oxidation by methanotrophic cultures from a groundwater aquifer. Appl Environ Microbiol. 1991 Jan;57(1):236–244. doi: 10.1128/aem.57.1.236-244.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Janssen D. B., Scheper A., Dijkhuizen L., Witholt B. Degradation of halogenated aliphatic compounds by Xanthobacter autotrophicus GJ10. Appl Environ Microbiol. 1985 Mar;49(3):673–677. doi: 10.1128/aem.49.3.673-677.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Nelson M. J., Montgomery S. O., Mahaffey W. R., Pritchard P. H. Biodegradation of trichloroethylene and involvement of an aromatic biodegradative pathway. Appl Environ Microbiol. 1987 May;53(5):949–954. doi: 10.1128/aem.53.5.949-954.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Nelson M. J., Montgomery S. O., O'neill E. J., Pritchard P. H. Aerobic metabolism of trichloroethylene by a bacterial isolate. Appl Environ Microbiol. 1986 Aug;52(2):383–384. doi: 10.1128/aem.52.2.383-384.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Nelson M. J., Montgomery S. O., Pritchard P. H. Trichloroethylene metabolism by microorganisms that degrade aromatic compounds. Appl Environ Microbiol. 1988 Feb;54(2):604–606. doi: 10.1128/aem.54.2.604-606.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Oldenhuis R., Oedzes J. Y., van der Waarde J. J., Janssen D. B. Kinetics of chlorinated hydrocarbon degradation by Methylosinus trichosporium OB3b and toxicity of trichloroethylene. Appl Environ Microbiol. 1991 Jan;57(1):7–14. doi: 10.1128/aem.57.1.7-14.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Oldenhuis R., Vink R. L., Janssen D. B., Witholt B. Degradation of chlorinated aliphatic hydrocarbons by Methylosinus trichosporium OB3b expressing soluble methane monooxygenase. Appl Environ Microbiol. 1989 Nov;55(11):2819–2826. doi: 10.1128/aem.55.11.2819-2826.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Strand S. E., Shippert L. Oxidation of chloroform in an aerobic soil exposed to natural gas. Appl Environ Microbiol. 1986 Jul;52(1):203–205. doi: 10.1128/aem.52.1.203-205.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Tsien H. C., Brusseau G. A., Hanson R. S., Waclett L. P. Biodegradation of trichloroethylene by Methylosinus trichosporium OB3b. Appl Environ Microbiol. 1989 Dec;55(12):3155–3161. doi: 10.1128/aem.55.12.3155-3161.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. van den Wijngaard A. J., Wind R. D., Janssen D. B. Kinetics of bacterial growth on chlorinated aliphatic compounds. Appl Environ Microbiol. 1993 Jul;59(7):2041–2048. doi: 10.1128/aem.59.7.2041-2048.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]

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