Skip to main content
The American Journal of Pathology logoLink to The American Journal of Pathology
. 1978 Apr;91(1):155–174.

Autofluorescent particles of human uterine muscle cells.

R G Gosden, H K Hawkins, C A Gosden
PMCID: PMC2018162  PMID: 645817

Abstract

Smooth muscle tissue collected from the uterine fundus of 24 patients undergoing hysterectomy was examined for chromolipoid pigments by histochemical and electron microscopic techniques. Certain cytoplasmic particles were found, mainly in smooth muscle cells, which exhibited characteristic autofluorescence, sudanophilia, and acid phosphatase activity but did not correspond to any typical pigment described previously. These particles were present in all subjects and they tended to increase in number with age. Chemical tests on tissue lipid extracts failed to prove that vitamin A was responsible for the fluorescence. The ultrastructural appearance of the particles somewhat variable, but most particles were rounded and of low electron density, with a lucent central space and dense bodies, probably lysosomes, at the periphery. The whole complex was enclosed by a single trilaminar membrane.

Full text

PDF
155

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bal H. S., Getty R. Changes in the histomorphology of the uterus of the domestic pig (Sus scrofa domesticus) with advancing age. J Gerontol. 1973 Apr;28(2):160–172. doi: 10.1093/geronj/28.2.160. [DOI] [PubMed] [Google Scholar]
  2. Desai I. D., Fletcher B. L., Tappel A. L. Fluorescent pigments from uterus of vitamin E-deficient rats. Lipids. 1975 May;10(5):307–309. doi: 10.1007/BF02532706. [DOI] [PubMed] [Google Scholar]
  3. Dessouky D. A. Myometrical changes in postpartum uterine involution. Am J Obstet Gynecol. 1971 Jun 1;110(3):318–329. doi: 10.1016/0002-9378(71)90721-6. [DOI] [PubMed] [Google Scholar]
  4. ESSNER E., NOVIKOFF A. B. Human hepatocellular pigments and lysosomes. J Ultrastruct Res. 1960 Jun;3:374–391. doi: 10.1016/s0022-5320(60)90016-2. [DOI] [PubMed] [Google Scholar]
  5. FULLERTON P. M. Pigmentation of jejunal muscle. Br Med J. 1960 Jan 23;1(5168):249–251. doi: 10.1136/bmj.1.5168.249. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Fletcher B. L., Dillard C. J., Tappel A. L. Measurement of fluorescent lipid peroxidation products in biological systems and tissues. Anal Biochem. 1973 Mar;52(1):1–9. doi: 10.1016/0003-2697(73)90327-8. [DOI] [PubMed] [Google Scholar]
  7. GEER J. C., McGILL H. C., Jr, STRONG J. P. The fine structure of human atherosclerotic lesions. Am J Pathol. 1961 Mar;38:263–287. [PMC free article] [PubMed] [Google Scholar]
  8. GRESHAM G. A., CRUICKSHANK J. G., VALENTINE J. C. Pigmentation of intestinal muscle in steatorrhoea. Nature. 1958 Feb 22;181(4608):538–539. doi: 10.1038/181538a0. [DOI] [PubMed] [Google Scholar]
  9. Graham C. E. Lipofuscin: identification and localization in monkey uterus. Am J Obstet Gynecol. 1968 Oct 15;102(4):490–492. doi: 10.1016/0002-9378(68)90528-0. [DOI] [PubMed] [Google Scholar]
  10. LAGUENS R., LAGRUTTA J. FINE STRUCTURE OF HUMAN UTERINE MUSCLE IN PREGNANCY. Am J Obstet Gynecol. 1964 Aug 15;89:1040–1047. doi: 10.1016/0002-9378(64)90296-0. [DOI] [PubMed] [Google Scholar]
  11. LOBEL B. L., DEANE H. W. Enzymic activity associated with postpartum involution of the uterus and with its regression after hormone withdrawal in the rat. Endocrinology. 1962 Apr;70:567–578. doi: 10.1210/endo-70-4-567. [DOI] [PubMed] [Google Scholar]
  12. LUFT J. H. Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol. 1961 Feb;9:409–414. doi: 10.1083/jcb.9.2.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lo Stumbo F. Ultrastruttura della cellulá muscolare dell'utero umano in situazioni fisiologiche diverse. I. Nell'utero normale non gravido. Riv Ostet Ginecol. 1965 Jul;20(7):436–452. [PubMed] [Google Scholar]
  14. Marshall J. R., Adams J. G., O'Neal R. M., De Bakey M. E. The ultrastructure of uncomplicated human atheroma in surgically resected aortas. J Atheroscler Res. 1966 Mar-Apr;6(2):120–131. doi: 10.1016/s0368-1319(66)80017-0. [DOI] [PubMed] [Google Scholar]
  15. NEELD J. B., Jr, PEARSON W. N. Macro- and micromethods for the determination of serum vitamin A using trifluoroacetic acid. J Nutr. 1963 Apr;79:454–462. doi: 10.1093/jn/79.4.454. [DOI] [PubMed] [Google Scholar]
  16. Nye S. W., Chittayasothorn K. Ceroid in the gastrointestinal smooth muscle of the Thai-Lao ethnic group. Am J Pathol. 1967 Aug;51(2):287–299. [PMC free article] [PubMed] [Google Scholar]
  17. Parker F., Odland G. F. A correlative histochemical, biochemical and electron microscopic study of experimental atherosclerosis in the rabbit aorta with special reference to the myo-intimal cell. Am J Pathol. 1966 Feb;48(2):197–239. [PMC free article] [PubMed] [Google Scholar]
  18. Scott R. F., Jones R., Daoud A. S., Zumbo O., Coulston F., Thomas W. A. Experimental atherosclerosis in rhesus monkeys. II. Cellular elements of proliferative lesions and possible role of cytoplasmic degeneration in pathogenesis as studied by electron microscopy. Exp Mol Pathol. 1967 Aug;7(1):34–57. doi: 10.1016/0014-4800(67)90037-8. [DOI] [PubMed] [Google Scholar]
  19. Shio H., Farquhar M. G., de Duve C. Lysosomes of the arterial wall. IV. Cytochemical localization of acid phosphatase and catalase in smooth muscle cells and foam cells from rabbit atheromatous aorta. Am J Pathol. 1974 Jul;76(1):1–16. [PMC free article] [PubMed] [Google Scholar]
  20. THOMPSON S. W., 2nd Leiomyometaplasts, particles of unknown significance in smooth muscle cells. Am J Pathol. 1958 Mar-Apr;34(2):273–283. [PMC free article] [PubMed] [Google Scholar]

Articles from The American Journal of Pathology are provided here courtesy of American Society for Investigative Pathology

RESOURCES