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. 1994 Sep;60(9):3419–3420. doi: 10.1128/aem.60.9.3419-3420.1994

Deconjugation of bile acids by lactobacilli in the mouse small bowel.

G W Tannock 1, A Tangerman 1, A Van Schaik 1, M A McConnell 1
PMCID: PMC201821  PMID: 7944373

Abstract

Conjugated and unconjugated bile acid concentrations in the small bowel contents and portal serum samples collected from mice with and without lactobacilli (RLFL and RLF, respectively) as gastrointestinal inhabitants were measured. The major portion of bile acids in the small bowel contents of RLFL mice was unconjugated (67.9%) in contrast to that of RFL animals in which a smaller portion of bile acids was unconjugated (23.5%). This study demonstrated that bile salt hydrolase produced by lactobacilli is active under the conditions prevailing in the proximal small bowels of mice.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bolt M. J., Stellaard F., Sitrin M. D., Paumgartner G. Serum unconjugated bile acids in patients with small bowel bacterial overgrowth. Clin Chim Acta. 1989 Apr 28;181(1):87–101. doi: 10.1016/0009-8981(89)90321-5. [DOI] [PubMed] [Google Scholar]
  2. Feighner S. D., Dashkevicz M. P. Subtherapeutic levels of antibiotics in poultry feeds and their effects on weight gain, feed efficiency, and bacterial cholyltaurine hydrolase activity. Appl Environ Microbiol. 1987 Feb;53(2):331–336. doi: 10.1128/aem.53.2.331-336.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Fuller R., Barrow P. A., Brooker B. E. Bacteria associated with the gastric epithelium of neonatal pigs. Appl Environ Microbiol. 1978 Mar;35(3):582–591. doi: 10.1128/aem.35.3.582-591.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Fuller R., Brooker B. E. Lactobacilli which attach to the crop epithelium of the fowl. Am J Clin Nutr. 1974 Nov;27(11):1305–1312. doi: 10.1093/ajcn/27.11.1305. [DOI] [PubMed] [Google Scholar]
  5. Masclee A., Tangerman A., van Schaik A., van der Hoek E. W., van Tongeren J. H. Unconjugated serum bile acids as a marker of small intestinal bacterial overgrowth. Eur J Clin Invest. 1989 Aug;19(4):384–389. doi: 10.1111/j.1365-2362.1989.tb00246.x. [DOI] [PubMed] [Google Scholar]
  6. McConnell M. A., Tannock G. W. A note on lactobacilli and beta-glucuronidase activity in the intestinal contents of mice. J Appl Bacteriol. 1993 Jun;74(6):649–651. doi: 10.1111/j.1365-2672.1993.tb05198.x. [DOI] [PubMed] [Google Scholar]
  7. McConnell M. A., Tannock G. W. Lactobacilli and azoreductase activity in the murine cecum. Appl Environ Microbiol. 1991 Dec;57(12):3664–3665. doi: 10.1128/aem.57.12.3664-3665.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Salemans J. M., Nagengast F. M., Tangerman A., van Schaik A., Hopman W. P., de Haan A. F., Jansen J. B. Effect of ageing on postprandial conjugated and unconjugated serum bile acid levels in healthy subjects. Eur J Clin Invest. 1993 Mar;23(3):192–198. doi: 10.1111/j.1365-2362.1993.tb00761.x. [DOI] [PubMed] [Google Scholar]
  9. Savage D. C., Dubos R., Schaedler R. W. The gastrointestinal epithelium and its autochthonous bacterial flora. J Exp Med. 1968 Jan 1;127(1):67–76. doi: 10.1084/jem.127.1.67. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Tangerman A., van Schaik A., van der Hoek E. W. Analysis of conjugated and unconjugated bile acids in serum and jejunal fluid of normal subjects. Clin Chim Acta. 1986 Sep 15;159(2):123–132. doi: 10.1016/0009-8981(86)90044-6. [DOI] [PubMed] [Google Scholar]
  11. Tannock G. W., Crichton C., Welling G. W., Koopman J. P., Midtvedt T. Reconstitution of the gastrointestinal microflora of lactobacillus-free mice. Appl Environ Microbiol. 1988 Dec;54(12):2971–2975. doi: 10.1128/aem.54.12.2971-2975.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Tannock G. W., Dashkevicz M. P., Feighner S. D. Lactobacilli and bile salt hydrolase in the murine intestinal tract. Appl Environ Microbiol. 1989 Jul;55(7):1848–1851. doi: 10.1128/aem.55.7.1848-1851.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]

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