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. 1978 Jun;91(3):581–594.

Motility of human polymorphonuclear leukocytes. Roles of hydroxy fatty acids, other lipids, and cations.

W S Lynn, C Mukherjee
PMCID: PMC2018302  PMID: 655264

Abstract

Rapid ionic fluxes across local areas of leukocyte plasma membranes result in local swelling, ie, ruffling or blebbing. These fluxes are stimulated either locally or generally over the cell membranes by hydrophobic chemotactic peptides. This swelling is associated with migration of the cells in the direction of the swollen area of the cell. Hydroxy fatty acids, other lipids, and Ca++ ionophores activate a Ca++-dependent migratory activity of the cell, whereas acidic peptides activate a monovalent cation-dependent migratory activity. These two processes are therefore additive. The swelling due to the peptides results in an increase in urea space but no change in inulin space, whereas, swelling due to permeant cations and anions causes a massive increase in both spaces. Migration appears to result from the pushing of cellular contents, nucleus and cytoplasm, into the more swollen area of the cell by unopposed contraction of the unswollen area of the cell.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ash J. F., Singer S. J. Concanavalin-A-induced transmembrane linkage of concanavalin A surface receptors to intracellular myosin-containing filaments. Proc Natl Acad Sci U S A. 1976 Dec;73(12):4575–4579. doi: 10.1073/pnas.73.12.4575. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Aswanikumar S., Schiffmann E., Corcoran B. A., Wahl S. M. Role of a peptidase in phagocyte chemotaxis. Proc Natl Acad Sci U S A. 1976 Jul;73(7):2439–2442. doi: 10.1073/pnas.73.7.2439. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Becker E. L., Davis A. T., Estensen R. D., Quie P. G. Cytochalasin B. IV. Inhibition and stimulation of chemotaxis of rabbit and human polymorphonuclear leukocytes. J Immunol. 1972 Feb;108(2):396–402. [PubMed] [Google Scholar]
  4. Boucek M. M., Snyderman R. Calcium influx requirement for human neutrophil chemotaxis: inhibition by lanthanum chloride. Science. 1976 Sep 3;193(4256):905–907. doi: 10.1126/science.948752. [DOI] [PubMed] [Google Scholar]
  5. Chan H. W., Prescott F. A. Specificity of lipoxygenases. Separation of isomeric hydroperoxides by high performance liquid chromatography. Biochim Biophys Acta. 1975 Jan 24;380(1):141–144. [PubMed] [Google Scholar]
  6. Gallin E. K., Wiederhold M. L., Lipsky P. E., Rosenthal A. S. Spontaneous and induced membrane hyperpolarizations in macrophages. J Cell Physiol. 1975 Dec;86 (Suppl 2)(3 Pt 2):653–661. doi: 10.1002/jcp.1040860510. [DOI] [PubMed] [Google Scholar]
  7. Gallin J. I., Rosenthal A. S. The regulatory role of divalent cations in human granulocyte chemotaxis. Evidence for an association between calcium exchanges and microtubule assembly. J Cell Biol. 1974 Sep;62(3):594–609. doi: 10.1083/jcb.62.3.594. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Goetzl E. J., Woods J. M., Gorman R. R. Stimulation of human eosinophil and neutrophil polymorphonuclear leukocyte chemotaxis and random migration by 12-L-hydroxy-5,8,10,14-eicosatetraenoic acid. J Clin Invest. 1977 Jan;59(1):179–183. doi: 10.1172/JCI108617. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hartwig J. H., Stossel T. P. Interactions of actin, myosin, and an actin-binding protein of rabbit pulmonary macrophages. III. Effects of cytochalasin B. J Cell Biol. 1976 Oct;71(1):295–303. doi: 10.1083/jcb.71.1.295. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hsu L. S., Becker E. L. Volume changes induced in rabbit polymorphonuclear leukocytes by chemotactic factor and cytochalasin B. Am J Pathol. 1975 Oct;81(1):1–14. [PMC free article] [PubMed] [Google Scholar]
  11. Kaplan A. P., Kay A. B., Austen K. F. A prealbumin activator of prekallikrein. 3. Appearance of chemotactic activity for human neutrophils by the conversion of human prekallikrein to kallikrein. J Exp Med. 1972 Jan;135(1):81–97. doi: 10.1084/jem.135.1.81. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Malech H. L., Root R. K., Gallin J. I. Structural analysis of human neutrophil migration. Centriole, microtubule, and microfilament orientation and function during chemotaxis. J Cell Biol. 1977 Dec;75(3):666–693. doi: 10.1083/jcb.75.3.666. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. McClatchey W., Snyderman R. Prostaglandins and inflammation: enhancement of monocyte chemotactic responsiveness by prostaglandin E2. Prostaglandins. 1976 Sep;12(3):415–426. doi: 10.1016/0090-6980(76)90022-8. [DOI] [PubMed] [Google Scholar]
  14. Miranda A. F., Godman G. C., Tanenbaum S. W. Action of cytochalasin D on cells of established lines. II. Cortex and microfilaments. J Cell Biol. 1974 Aug;62(2):406–423. doi: 10.1083/jcb.62.2.406. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Naccache P. H., Showell H. J., Becker E. L., Sha'afi R. I. Transport of sodium, potassium, and calcium across rabbit polymorphonuclear leukocyte membranes. Effect of chemotactic factor. J Cell Biol. 1977 May;73(2):428–444. doi: 10.1083/jcb.73.2.428. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Nugteren D. H. Arachidonate lipoxygenase in blood platelets. Biochim Biophys Acta. 1975 Feb 20;380(2):299–307. doi: 10.1016/0005-2760(75)90016-8. [DOI] [PubMed] [Google Scholar]
  17. Pichard A. L., Cheung W. Y. Cyclic 3':5'-nucleotide phosphodiesterase. Stimulation of bovine brain cytoplasmic enzyme by lysophosphatidylcholine. J Biol Chem. 1977 Jul 25;252(14):4872–4875. [PubMed] [Google Scholar]
  18. Puck T. T. Cyclic AMP, the microtubule-microfilament system, and cancer. Proc Natl Acad Sci U S A. 1977 Oct;74(10):4491–4495. doi: 10.1073/pnas.74.10.4491. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Shibuya E., Masuda K., Izawa Y. Effects of prostaglandins on leukocyte migration. Prostaglandins. 1976 Aug;12(2):165–174. doi: 10.1016/0090-6980(76)90110-6. [DOI] [PubMed] [Google Scholar]
  20. Shin H. S., Snyderman R., Friedman E., Mellors A., Mayer M. M. Chemotactic and anaphylatoxic fragment cleaved from the fifth component of guinea pig complement. Science. 1968 Oct 18;162(3851):361–363. doi: 10.1126/science.162.3851.361. [DOI] [PubMed] [Google Scholar]
  21. Showell H. J., Becker E. L. The effects of external K+ and Na+ on the chemotaxis of rabbit peritoneal neutrophils. J Immunol. 1976 Jan;116(1):99–105. [PubMed] [Google Scholar]
  22. Showell H. J., Freer R. J., Zigmond S. H., Schiffmann E., Aswanikumar S., Corcoran B., Becker E. L. The structure-activity relations of synthetic peptides as chemotactic factors and inducers of lysosomal secretion for neutrophils. J Exp Med. 1976 May 1;143(5):1154–1169. doi: 10.1084/jem.143.5.1154. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Spilbert I., Gallacher A., Mehta J. M., Mandell B. Urate crystal-induced chemotactic factor: isolation and partial characterization. J Clin Invest. 1976 Oct;58(4):815–819. doi: 10.1172/JCI108533. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Turner S. R., Tainer J. A., Lynn W. S. Biogenesis of chemotactic molecules by the arachidonate lipoxygenase system of platelets. Nature. 1975 Oct 23;257(5528):680–681. doi: 10.1038/257680a0. [DOI] [PubMed] [Google Scholar]
  25. Ward P. A., Becker E. L. Biochemical demonstration of the activatable esterase of the rabbit netrophil involved in the chemotactic response. J Immunol. 1970 Nov;105(5):1057–1067. [PubMed] [Google Scholar]
  26. Zigmond S. H. Mechanisms of sensing chemical gradients by polymorphonuclear leukocytes. Nature. 1974 May 31;249(456):450–452. doi: 10.1038/249450a0. [DOI] [PubMed] [Google Scholar]

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