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. 1994 Sep;60(9):3466–3469. doi: 10.1128/aem.60.9.3466-3469.1994

Biodegradation of 2-nitrotoluene by Pseudomonas sp. strain JS42.

B E Haigler 1, W H Wallace 1, J C Spain 1
PMCID: PMC201834  PMID: 7944378

Abstract

A strain of Pseudomonas sp. was isolated from nitrobenzene-contaminated soil and groundwater on 2-nitrotoluene as the sole source of carbon, energy, and nitrogen. Bacterial cells growing on 2-nitrotoluene released nitrite into the growth medium. The isolate also grew on 3-methylcatechol, 4-methylcatechol, and catechol. 2-Nitrotoluene, 3-methylcatechol, and catechol stimulated oxygen consumption by intact cells regardless of the growth substrate. Crude extracts from the isolate contained catechol 2,3-dioxygenase and 2-hydroxy-6-oxohepta-2,4-dienoate hydrolase activity. The results suggest that 2-nitrotoluene is subject to initial attack by a dioxygenase enzyme that forms 3-methylcatechol with concomitant release of nitrite. The 3-methylcatechol is subsequently degraded via the meta ring fission pathway.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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