Skip to main content
NCBI home page
The American Journal of Pathology logoLink to The American Journal of Pathology
. 1978 Jul;92(1):85–98.

Relationship between glomerular mesangial cell proliferation and amyloid deposition as seen by ultrastructural and morphometric analysis in experimental kala-azar of the hamster.

M I Duarte, A Sesso, T de Brito
PMCID: PMC2018592  PMID: 686150

Abstract

Light and electron microscopic studies combined with a morphometric analysis of the hamster glomerulus in experimental kala-azar showed progressive hyperplasia of mesangial cells beginning on the 10th day and reaching a peak on the 20th day after infection. Afterward, the number of mesangial cells declined and a progressive rise of amyloid deposits over the mesangial matrix was observed. This system for amyloid production is unique if we consider that probably one cell, the mesangial cell, is involved in glomerular amyloid deposition. Our data support a slight modification in the sequence of events of the biphasic theory of amyloid formation. We observed that the number of mesangial cells declines when amyloid deposition increases and that mesangial cell morphology in this stage is not that of an actively secreting cell. It is therefore hypothesized that amyloid precursor material is secreted into the matrix during the proliferative phase. In the second phase, amyloid deposits occur in the extracellular media close to functionally impaired mesangial cells.

Full text

PDF
85

Images in this article

96Figure 2
on p.96
98Figure 4
on p.98
95Figure 1
on p.95
97Figure 3
on p.97

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BACH G. UBER DIE BESTIMMUNG VON CHARAKTERISTISCHEN GROESSEN EINER KUGELVERTEILUNG AUS DER VERTEILUNG DER SCHNITTKREISE. Z Wiss Mikrosk. 1963 Sep;65:285–291. [PubMed] [Google Scholar]
  2. Ben-Ishay Z., Zlotnick A. The cellular origin of amyloid. Electron microscopid study in a case of amyloidosis. Isr J Med Sci. 1968 Sep-Oct;4(5):987–994. [PubMed] [Google Scholar]
  3. Bryceson A. D. Immunological aspects of clinical leishmaniasis. Proc R Soc Med. 1970 Oct;63(10):1056–1060. doi: 10.1177/003591577006301048. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. COHEN A. S., CALKINS E. A study of the fine structure of the kidney in casein-induced amyloidosis in rabbits. J Exp Med. 1960 Sep 1;112:479–490. doi: 10.1084/jem.112.3.479. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cohen A. S. Amyloidosis. N Engl J Med. 1967 Sep 7;277(10):522–contd. doi: 10.1056/NEJM196709072771006. [DOI] [PubMed] [Google Scholar]
  6. GUEFT B., GHIDONI J. J. THE SITE OF FORMATION AND ULTRASTRUCTURE OF AMYLOID. Am J Pathol. 1963 Nov;43:837–854. [PMC free article] [PubMed] [Google Scholar]
  7. HINGLAIS N., DE MONTERA H. ETUDE AU MICROSCOPE 'ELECTRONIQUE DE SIX CAS D'AMYLOSE R'ENALE HUMAINE. Pathol Biol. 1964 Feb;12:176–191. [PubMed] [Google Scholar]
  8. HINGLAIS N., ZWEIBAUM A., RICHET G. LES L'ESIONS PR'ECOCES DE L'AMYLOSE EXP'ERIMENTALE DU HAMSTER. ETUDE AU MICROSCOPE ELECTRONIQUE. Nephron. 1964;1:16–30. doi: 10.1159/000179316. [DOI] [PubMed] [Google Scholar]
  9. HJORT G. H., CHRISTENSEN H. E. Electron-microscopic investigations on secondary renal amyloidosis. Acta Rheumatol Scand. 1961;7:65–68. doi: 10.3109/rhe1.1961.7.issue-1-4.15. [DOI] [PubMed] [Google Scholar]
  10. LUPASCO G., AGAVRILOAIEI A., CIPLEA A. G. [Contribution to the study of the pathogenesis and histopathology of Leishmania donovani infection in the golden hamster (Cricetus auratus)]. Arch Roum Pathol Exp Microbiol. 1961 Dec;20:565–578. [PubMed] [Google Scholar]
  11. MOVAT H. Z. The fine structure of the glomerulus in amyloidosis. Arch Pathol. 1960 Mar;69:323–332. [PubMed] [Google Scholar]
  12. Meleney H. E. The Histopathology of Kala-Azar in the Hamster, Monkey, and Man. Am J Pathol. 1925 Mar;1(2):147–168.11. [PMC free article] [PubMed] [Google Scholar]
  13. Ranlov P., Wanstrup J. Ultrastructural investigations on the cellular morphogenesis of experimental mouse amyloidosis. Acta Pathol Microbiol Scand. 1967;71(4):575–591. doi: 10.1111/j.1699-0463.1967.tb05178.x. [DOI] [PubMed] [Google Scholar]
  14. SHIMAMURA T., SORENSON G. D. EXPERIMENTAL AMYLOIDOSIS.V. RELATIONSHIP BETWEEN EXPERIMENTAL GLOMERULAR AMYLOID AND THE MESANGIAL REGION. Am J Pathol. 1965 Apr;46:645–656. [PMC free article] [PubMed] [Google Scholar]
  15. Shirahama T., Cohen A. S. Lysosomal breakdown of amyloid fibrils by macrophages. Am J Pathol. 1971 Jun;63(3):463–486. [PMC free article] [PubMed] [Google Scholar]
  16. Suzuki Y., Churg J., Grishman E., Mautner W., Dachs S. The Mesangium of the Renal Glomerulus: Electron Microscopic Studies of Pathologic Alterations. Am J Pathol. 1963 Oct;43(4):555–578. [PMC free article] [PubMed] [Google Scholar]
  17. TEILUM G. PATHOGENESIS OF AMYLOIDOSIS. Acta Pathol Microbiol Scand. 1964;61:21–45. doi: 10.1111/apm.1964.61.1.21. [DOI] [PubMed] [Google Scholar]
  18. TEILUM G. Periodic acid-Schiff-positive reticulo-endothelial cells producing glycoprotein; functional significance during formation of amyloid. Am J Pathol. 1956 Sep-Oct;32(5):945–959. [PMC free article] [PubMed] [Google Scholar]
  19. Uchino F. Pathological study on amyloidosis. Role of reticuloendothelial cells in inducing amyloidosis. Acta Pathol Jpn. 1967 Feb;17(1):49–82. [PubMed] [Google Scholar]
  20. WEIBEL E. R., GOMEZ D. M. A principle for counting tissue structures on random sections. J Appl Physiol. 1962 Mar;17:343–348. doi: 10.1152/jappl.1962.17.2.343. [DOI] [PubMed] [Google Scholar]
  21. Weibel E. R. Stereological principles for morphometry in electron microscopic cytology. Int Rev Cytol. 1969;26:235–302. doi: 10.1016/s0074-7696(08)61637-x. [DOI] [PubMed] [Google Scholar]
  22. Zucker-Franklin D., Franklin E. C. Intracellular localization of human amyloid by fluorescence and electron microscopy. Am J Pathol. 1970 Apr;59(1):23–41. [PMC free article] [PubMed] [Google Scholar]
  23. Zòltowska A., Wrzolkowa T. Experimental amyloidosis in hamsters. J Pathol. 1973 Feb;109(2):93–99. doi: 10.1002/path.1711090203. [DOI] [PubMed] [Google Scholar]

Articles from The American Journal of Pathology are provided here courtesy of American Society for Investigative Pathology

RESOURCES