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. 1994 Oct;60(10):3867–3869. doi: 10.1128/aem.60.10.3867-3869.1994

A transcriptional reporter for in vivo promoter analysis in the archaeon Haloferax volcanii.

J R Palmer 1, C J Daniels 1
PMCID: PMC201898  PMID: 7986055

Abstract

We have used a modified intron-containing tRNA(Pro(UGG) gene (tRNA(ProM), derived from the Saccharomyces cerevisiae tRNA(Pro(UGG) gene, as a reporter to measure in vivo transcription from a halophilic archaeon promoter. Coupling of the yeast tRNA(ProM) gene to the Haloferax volcanii tRNA(Lys) promoter on the H. volcanii plasmid pWL201 led to the production of a single stable transcript that was readily quantitated by Northern (RNA) blot analysis. Comparison of tRNA(ProM) RNA production from constructs containing the wild-type tRNA(Lys) promoter and those containing mutant tRNA(Lys) promoters demonstrated that this assay system can be used to measure expression from strong and weak promoters.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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