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. 1994 Oct;60(10):3870–3873. doi: 10.1128/aem.60.10.3870-3873.1994

Glucose uptake by Listeria monocytogenes Scott A and inhibition by pediocin JD.

D P Christensen 1, R W Hutkins 1
PMCID: PMC201899  PMID: 7986056

Abstract

Glucose uptake by Listeria monocytogenes Scott A was inhibited by the bacteriocin pediocin JD and by the protonophore carbonyl cyanide m-chlorophenyhydrazone. Experiments with monensin, nigericin, chlorhexidine diacetate, dinitrophenol, and gramicidin, however, showed that glucose uptake could occur in the absence of a proton motive force. L. monocytogenes cell extracts phosphorylated glucose when phosphoenolpyruvate (PEP) was present in the assay mixture, and whole cells incubated with 2-deoxyglucose accumulated 2-deoxyglucose-6-phosphate, indicating the presence of a PEP-dependent phosphotransferase system in this organism. Glucose phosphorylation also occurred when ATP was present, suggesting that a proton motive force-mediated glucose transport system may also be present. We conclude that L. monocytogenes Scott A accumulates glucose by phosphotransferase and proton motive force-mediated systems, both of which are sensitive to pediocin JD.

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Selected References

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  1. Abee T., Klaenhammer T. R., Letellier L. Kinetic studies of the action of lactacin F, a bacteriocin produced by Lactobacillus johnsonii that forms poration complexes in the cytoplasmic membrane. Appl Environ Microbiol. 1994 Mar;60(3):1006–1013. doi: 10.1128/aem.60.3.1006-1013.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bruno M. E., Kaiser A., Montville T. J. Depletion of proton motive force by nisin in Listeria monocytogenes cells. Appl Environ Microbiol. 1992 Jul;58(7):2255–2259. doi: 10.1128/aem.58.7.2255-2259.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bruno M. E., Montville T. J. Common mechanistic action of bacteriocins from lactic Acid bacteria. Appl Environ Microbiol. 1993 Sep;59(9):3003–3010. doi: 10.1128/aem.59.9.3003-3010.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chikindas M. L., García-Garcerá M. J., Driessen A. J., Ledeboer A. M., Nissen-Meyer J., Nes I. F., Abee T., Konings W. N., Venema G. Pediocin PA-1, a bacteriocin from Pediococcus acidilactici PAC1.0, forms hydrophilic pores in the cytoplasmic membrane of target cells. Appl Environ Microbiol. 1993 Nov;59(11):3577–3584. doi: 10.1128/aem.59.11.3577-3584.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Christensen D. P., Hutkins R. W. Collapse of the proton motive force in Listeria monocytogenes caused by a bacteriocin produced by Pediococcus acidilactici. Appl Environ Microbiol. 1992 Oct;58(10):3312–3315. doi: 10.1128/aem.58.10.3312-3315.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Farber J. M., Peterkin P. I. Listeria monocytogenes, a food-borne pathogen. Microbiol Rev. 1991 Sep;55(3):476–511. doi: 10.1128/mr.55.3.476-511.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gao F. H., Abee T., Konings W. N. Mechanism of action of the peptide antibiotic nisin in liposomes and cytochrome c oxidase-containing proteoliposomes. Appl Environ Microbiol. 1991 Aug;57(8):2164–2170. doi: 10.1128/aem.57.8.2164-2170.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hutkins R. W., Kashket E. R. Phosphotransferase Activity in Clostridium acetobutylicum from Acidogenic and Solventogenic Phases of Growth. Appl Environ Microbiol. 1986 May;51(5):1121–1123. doi: 10.1128/aem.51.5.1121-1123.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kashket E. R., Blanchard A. G., Metzger W. C. Proton motive force during growth of Streptococcus lactis cells. J Bacteriol. 1980 Jul;143(1):128–134. doi: 10.1128/jb.143.1.128-134.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kornberg H. L., Reeves R. E. Inducible phosphoenolpyruvate-dependent hexose phosphotransferase activities in Escherichia coli. Biochem J. 1972 Aug;128(5):1339–1344. doi: 10.1042/bj1281339. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Maftah A., Renault D., Vignoles C., Héchard Y., Bressollier P., Ratinaud M. H., Cenatiempo Y., Julien R. Membrane permeabilization of Listeria monocytogenes and mitochondria by the bacteriocin mesentericin Y105. J Bacteriol. 1993 May;175(10):3232–3235. doi: 10.1128/jb.175.10.3232-3235.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Martin S. A., Russell J. B. Transport and phosphorylation of disaccharides by the ruminal bacterium Streptococcus bovis. Appl Environ Microbiol. 1987 Oct;53(10):2388–2393. doi: 10.1128/aem.53.10.2388-2393.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Mitchell W. J., Reizer J., Herring C., Hoischen C., Saier M. H., Jr Identification of a phosphoenolpyruvate:fructose phosphotransferase system (fructose-1-phosphate forming) in Listeria monocytogenes. J Bacteriol. 1993 May;175(9):2758–2761. doi: 10.1128/jb.175.9.2758-2761.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Okereke A., Montville T. J. Nisin dissipates the proton motive force of the obligate anaerobe Clostridium sporogenes PA 3679. Appl Environ Microbiol. 1992 Aug;58(8):2463–2467. doi: 10.1128/aem.58.8.2463-2467.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Suputtamongkol Y., Kwiatkowski D., Dance D. A., Chaowagul W., White N. J. Tumor necrosis factor in septicemic melioidosis. J Infect Dis. 1992 Mar;165(3):561–564. doi: 10.1093/infdis/165.3.561. [DOI] [PubMed] [Google Scholar]
  16. Venema K., Abee T., Haandrikman A. J., Leenhouts K. J., Kok J., Konings W. N., Venema G. Mode of Action of Lactococcin B, a Thiol-Activated Bacteriocin from Lactococcus lactis. Appl Environ Microbiol. 1993 Apr;59(4):1041–1048. doi: 10.1128/aem.59.4.1041-1048.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Young K. M., Foegeding P. M. Acetic, lactic and citric acids and pH inhibition of Listeria monocytogenes Scott A and the effect on intracellular pH. J Appl Bacteriol. 1993 May;74(5):515–520. [PubMed] [Google Scholar]

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