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. 1994 Oct;60(10):3878–3880. doi: 10.1128/aem.60.10.3878-3880.1994

Characterization of a Protease from a Psychrotroph, Pseudomonas fluorescens 114

Tetsuo Hamamoto 1,*, Motohiro Kaneda 1,2, Koki Horikoshi 2, Toshiaki Kudo 1
PMCID: PMC201901  PMID: 16349422

Abstract

A psychrotrophic bacterium isolated from river sediment was identified as Pseudomonas fluorescens 114. It grew at 0°C and optimally at 20°C. The bacterium produced a protease with a molecular weight of 47,000, which was stable in the pH range of 5 to 9 and worked optimally between pH 6.5 and 10. Activity was optimal at 35°C and was lost immediately at 50°C and after 5 min at 45°C. At 0, 10, and 20°C, 24, 38, and 57% of optimal activity were observed, respectively.

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Selected References

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