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. 1994 Nov;60(11):4186–4188. doi: 10.1128/aem.60.11.4186-4188.1994

Correlation of bioenergetic parameters with cell death in Listeria monocytogenes cells exposed to nisin.

K Winkowski 1, M E Bruno 1, T J Montville 1
PMCID: PMC201958  PMID: 7993099

Abstract

In Listeria monocytogenes, nisin induced ATP efflux, reduced the intracellular ATP concentration within 1 min, and dissipated the proton motive force within 2 min. Efflux accounted for only 20% of the ATP depletion, suggesting that ATP hydrolysis also occurred. ATP efflux depended on nisin concentration and followed saturation kinetics. These results suggest that nisin breaches the membrane permeability barrier in a manner more consistent with pore formation than with a nonspecific detergent-like membrane destabilization.

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Selected References

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