Abstract
MilliQ water was inoculated with poliovirus type 1 strain LSc-1 and was treated with disinfectants, including chlorine, chlorine dioxide, ozone, and UV light. No relationship between probes and plaque assays were seen, demonstrating that viral nucleic acids were not destroyed. These findings suggest that nucleic acid probes cannot distinguish between infectious and noninfectious viruses and cannot be used in the evaluation of treated waters.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Alvarez M. E., O'Brien R. T. Mechanisms of inactivation of poliovirus by chlorine dioxide and iodine. Appl Environ Microbiol. 1982 Nov;44(5):1064–1071. doi: 10.1128/aem.44.5.1064-1071.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dahling D. R., Wright B. A. Optimization of the BGM cell line culture and viral assay procedures for monitoring viruses in the environment. Appl Environ Microbiol. 1986 Apr;51(4):790–812. doi: 10.1128/aem.51.4.790-812.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HUFF C. B., SMITH H. F., BORING W. D., CLARKE N. A. STUDY OF ULTRAVIOLET DISINFECTION OF WATER AND FACTORS IN TREATMENT EFFICIENCY. Public Health Rep. 1965 Aug;80:695–705. [PMC free article] [PubMed] [Google Scholar]
- Moore N. J., Margolin A. B. Evaluation of radioactive and nonradioactive gene probes and cell culture for detection of poliovirus in water samples. Appl Environ Microbiol. 1993 Sep;59(9):3145–3146. doi: 10.1128/aem.59.9.3145-3146.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ward R. L., Ashley C. S. Identification of the virucidal agent in wastewater sludge. Appl Environ Microbiol. 1977 Apr;33(4):860–864. doi: 10.1128/aem.33.4.860-864.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]