Abstract
We devised a new method (the dorayaki method) using marine agar under in situ pressures to isolate barophilic bacteria from the intestinal contents of three deep-sea fishes (two Coryphaenoides yaquinae samples and one Ilyophis sp. sample) retrieved from depths of 4,700 to 6,100 m in the Northwest Pacific Ocean. All 10 strains isolated from one sample (C. yaquinae) were obligately barophilic. One of the 10 strains did not grow at atmospheric pressure and 103.4 MPa but did grow well between 20.7 and 82.7 MPa, with optimal growth at 41.4 MPa. This method is useful for isolating psychrophilic and barophilic deep-sea bacteria.
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Selected References
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- Hobbie J. E., Daley R. J., Jasper S. Use of nuclepore filters for counting bacteria by fluorescence microscopy. Appl Environ Microbiol. 1977 May;33(5):1225–1228. doi: 10.1128/aem.33.5.1225-1228.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jannasch H. W., Wirsen C. O., Taylor C. D. Deep-sea bacteria: isolation in the absence of decompression. Science. 1982 Jun 18;216(4552):1315–1317. doi: 10.1126/science.216.4552.1315. [DOI] [PubMed] [Google Scholar]
- Yayanos A. A., Dietz A. S., VAN Boxtel R. Isolation of a deep-sea barophilic bacterium and some of its growth characteristics. Science. 1979 Aug 24;205(4408):808–810. doi: 10.1126/science.205.4408.808. [DOI] [PubMed] [Google Scholar]
- Yayanos A. A., Dietz A. S., Van Boxtel R. Dependence of reproduction rate on pressure as a hallmark of deep-sea bacteria. Appl Environ Microbiol. 1982 Dec;44(6):1356–1361. doi: 10.1128/aem.44.6.1356-1361.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yayanos A. A., Dietz A. S., Van Boxtel R. Obligately barophilic bacterium from the Mariana trench. Proc Natl Acad Sci U S A. 1981 Aug;78(8):5212–5215. doi: 10.1073/pnas.78.8.5212. [DOI] [PMC free article] [PubMed] [Google Scholar]