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. 1994 Dec;60(12):4580–4583. doi: 10.1128/aem.60.12.4580-4583.1994

Renibacterium salmoninarum, the causative agent of bacterial kidney disease in salmonid fish, detected by nested reverse transcription-PCR of 16S rRNA sequences.

H B Magnússon 1, O H Fridjónsson 1, O S Andrésson 1, E Benediktsdóttir 1, S Gudmundsdóttir 1, V Andrésdóttir 1
PMCID: PMC202022  PMID: 7529017

Abstract

An assay based on reverse transcription and nested PCR amplification of hypervariable regions within the 16S rRNA sequence was used to specifically detect Renibacterium salmoninarum, the slowly growing causative agent of bacterial kidney disease in salmonid fish. This assay detected 1 to 10 bacteria per sample and took 1 to 2 days to perform. The assay was used to detect R. salmoninarum in ovarian fluid obtained from naturally infected fish. The assay was unreliable when it was used to examine kidney tissue.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Böddinghaus B., Rogall T., Flohr T., Blöcker H., Böttger E. C. Detection and identification of mycobacteria by amplification of rRNA. J Clin Microbiol. 1990 Aug;28(8):1751–1759. doi: 10.1128/jcm.28.8.1751-1759.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. De Wit D., Steyn L., Shoemaker S., Sogin M. Direct detection of Mycobacterium tuberculosis in clinical specimens by DNA amplification. J Clin Microbiol. 1990 Nov;28(11):2437–2441. doi: 10.1128/jcm.28.11.2437-2441.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Fryer J. L., Sanders J. E. Bacterial kidney disease of salmonid fish. Annu Rev Microbiol. 1981;35:273–298. doi: 10.1146/annurev.mi.35.100181.001421. [DOI] [PubMed] [Google Scholar]
  4. Gutenberger S. K., Giovannoni S. J., Field K. G., Fryer J. L., Rohovec J. S. A phylogenetic comparison of the 16S rRNA sequence of the fish pathogen, Renibacterium salmoninarum, to gram-positive bacteria. FEMS Microbiol Lett. 1991 Jan 15;61(2-3):151–156. doi: 10.1016/0378-1097(91)90543-j. [DOI] [PubMed] [Google Scholar]
  5. Lane D. J., Pace B., Olsen G. J., Stahl D. A., Sogin M. L., Pace N. R. Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses. Proc Natl Acad Sci U S A. 1985 Oct;82(20):6955–6959. doi: 10.1073/pnas.82.20.6955. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Rehnstam A. S., Norqvist A., Wolf-Watz H., Hagström A. Identification of Vibrio anguillarum in fish by using partial 16S rRNA sequences and a specific 16S rRNA oligonucleotide probe. Appl Environ Microbiol. 1989 Aug;55(8):1907–1910. doi: 10.1128/aem.55.8.1907-1910.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Wilson K. H., Blitchington R., Shah P., McDonald G., Gilmore R. D., Mallavia L. P. Probe directed at a segment of Rickettsia rickettsii rRNA amplified with polymerase chain reaction. J Clin Microbiol. 1989 Dec;27(12):2692–2696. doi: 10.1128/jcm.27.12.2692-2696.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]

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