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. 1994 Dec;60(12):4624–4626. doi: 10.1128/aem.60.12.4624-4626.1994

Cloning of organic solvent tolerance gene ostA that determines n-hexane tolerance level in Escherichia coli.

R Aono 1, T Negishi 1, H Nakajima 1
PMCID: PMC202034  PMID: 7811102

Abstract

A variety of genes are involved in determining the level of organic solvent tolerance of Escherichia coli K-12. Gene ostA is one of the genes contributing to the level of organic solvent tolerance. This gene was cloned from an n-hexane-tolerant strain of E. coli, JA300. A JA300-based n-hexane-sensitive strain, OST4251, was converted to the n-hexane-tolerant phenotype by transformation with DNA containing the ostA gene derived from JA300. Thus, the cloned ostA gene complemented the n-hexane-sensitive phenotype of OST4251.

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Selected References

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