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. 1993 Jan;59(1):114–119. doi: 10.1128/aem.59.1.114-119.1993

Arbitrary primer polymerase chain reaction, a powerful method to identify Bacillus thuringiensis serovars and strains.

R Brousseau 1, A Saint-Onge 1, G Préfontaine 1, L Masson 1, J Cabana 1
PMCID: PMC202064  PMID: 8439143

Abstract

Arbitrary primer polymerase chain reaction technology has been applied to the identification of commercial strains of Bacillus thuringiensis by using total DNAs extracted from single bacterial colonies as templates. Characteristic DNA banding patterns can be readily and reproducibly obtained by agarose gel electrophoresis. This method has been used to distinguish commercial products containing B. thuringiensis serovar kurstaki (3a3b). When a single primer was used this method was capable of producing discriminating DNA fingerprints for 33 known serovars. Differentiation from the closely related species Bacillus cereus is also readily achieved. This technique should prove to be a powerful tool for identification and discrimination of individual B. thuringiensis strains.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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