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. 1993 Jan;59(1):316–321. doi: 10.1128/aem.59.1.316-321.1993

Strains of Corynebacterium glutamicum with Different Lysine Productivities May Have Different Lysine Excretion Systems

Stefan Bröer 1, Lothar Eggeling 1, Reinhard Krämer 1,*
PMCID: PMC202097  PMID: 16348855

Abstract

The lysine excretion systems of three different lysine-producing strains of Corynebacterium glutamicum were characterized in intact cells. Two strains (DG 52-5 and MH 20-22B) are lysine producers of different efficiency. They were bred by classical mutagenesis and have a feedback-resistant aspartate kinase. The third strain (KK 25) was constructed from the wild type by introducing the feedback-resistant aspartate kinase gene of strain MH 20-22B into its genome. The three strains were shown to possess different excretion systems. Export in strain KK 25 is much slower than in the two mutants. The differences between the two lysine-producing strains are more subtle. Km and Vmax are similar, but pH dependence and membrane potential dependence reveal differences in the intrinsic properties of the carrier system.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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