Abstract
A chitinase was purified from the culture filtrate of Streptomyces thermoviolaceus OPC-520. The enzyme showed a high optimum temperature (70 to 80 degrees C), a high optimum pH level (8.0 to 10.0), and heat stability. This enzyme showed high sequence homology with chitinases from Serratia marcescens QMB1466 and Bacillus circulans WL-12.
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- BERGER L. R., REYNOLDS D. M. The chitinase system of a strain of Streptomyces griseus. Biochim Biophys Acta. 1958 Sep;29(3):522–534. doi: 10.1016/0006-3002(58)90008-8. [DOI] [PubMed] [Google Scholar]
- Hara S., Yamamura Y., Fujii Y., Mega T., Ikenaka T. Purification and characterization of chitinase produced by Streptomyces erythraeus. J Biochem. 1989 Mar;105(3):484–489. doi: 10.1093/oxfordjournals.jbchem.a122691. [DOI] [PubMed] [Google Scholar]
- Jones J. D., Grady K. L., Suslow T. V., Bedbrook J. R. Isolation and characterization of genes encoding two chitinase enzymes from Serratia marcescens. EMBO J. 1986 Mar;5(3):467–473. doi: 10.1002/j.1460-2075.1986.tb04235.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kamei K., Yamamura Y., Hara S., Ikenaka T. Amino acid sequence of chitinase from Streptomyces erythraeus. J Biochem. 1989 Jun;105(6):979–985. doi: 10.1093/oxfordjournals.jbchem.a122791. [DOI] [PubMed] [Google Scholar]
- Kuranda M. J., Robbins P. W. Chitinase is required for cell separation during growth of Saccharomyces cerevisiae. J Biol Chem. 1991 Oct 15;266(29):19758–19767. [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
- Metraux J. P., Burkhart W., Moyer M., Dincher S., Middlesteadt W., Williams S., Payne G., Carnes M., Ryals J. Isolation of a complementary DNA encoding a chitinase with structural homology to a bifunctional lysozyme/chitinase. Proc Natl Acad Sci U S A. 1989 Feb;86(3):896–900. doi: 10.1073/pnas.86.3.896. [DOI] [PMC free article] [PubMed] [Google Scholar]
- RUPLEY J. A. THE HYDROLYSIS OF CHITIN BY CONCENTRATED HYDROCHLORIC ACID, AND THE PREPARATION OF LOW-MOLECULAR-WEIGHT SUBSTRATES FOR LYSOZYME. Biochim Biophys Acta. 1964 Nov 1;83:245–255. doi: 10.1016/0926-6526(64)90001-1. [DOI] [PubMed] [Google Scholar]
- Robbins P. W., Albright C., Benfield B. Cloning and expression of a Streptomyces plicatus chitinase (chitinase-63) in Escherichia coli. J Biol Chem. 1988 Jan 5;263(1):443–447. [PubMed] [Google Scholar]
- Robbins P. W., Overbye K., Albright C., Benfield B., Pero J. Cloning and high-level expression of chitinase-encoding gene of Streptomyces plicatus. Gene. 1992 Feb 1;111(1):69–76. doi: 10.1016/0378-1119(92)90604-n. [DOI] [PubMed] [Google Scholar]
- Shinshi H., Mohnen D., Meins F. Regulation of a plant pathogenesis-related enzyme: Inhibition of chitinase and chitinase mRNA accumulation in cultured tobacco tissues by auxin and cytokinin. Proc Natl Acad Sci U S A. 1987 Jan;84(1):89–93. doi: 10.1073/pnas.84.1.89. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Suzuki K., Tokoro A., Okawa Y., Suzuki S., Suzuki M. Effect of N-acetylchito-oligosaccharides on activation of phagocytes. Microbiol Immunol. 1986;30(8):777–787. doi: 10.1111/j.1348-0421.1986.tb03004.x. [DOI] [PubMed] [Google Scholar]
- Takayanagi T., Ajisaka K., Takiguchi Y., Shimahara K. Isolation and characterization of thermostable chitinases from Bacillus licheniformis X-7u. Biochim Biophys Acta. 1991 Jul 12;1078(3):404–410. doi: 10.1016/0167-4838(91)90163-t. [DOI] [PubMed] [Google Scholar]
- Tokoro A., Tatewaki N., Suzuki K., Mikami T., Suzuki S., Suzuki M. Growth-inhibitory effect of hexa-N-acetylchitohexaose and chitohexaose against Meth-A solid tumor. Chem Pharm Bull (Tokyo) 1988 Feb;36(2):784–790. doi: 10.1248/cpb.36.784. [DOI] [PubMed] [Google Scholar]
- Tsujibo H., Miyamoto K., Kuda T., Minami K., Sakamoto T., Hasegawa T., Inamori Y. Purification, properties, and partial amino acid sequences of thermostable xylanases from Streptomyces thermoviolaceus OPC-520. Appl Environ Microbiol. 1992 Jan;58(1):371–375. doi: 10.1128/aem.58.1.371-375.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Usui T., Hayashi Y., Nanjo F., Sakai K., Ishido Y. Transglycosylation reaction of a chitinase purified from Nocardia orientalis. Biochim Biophys Acta. 1987 Feb 20;923(2):302–309. doi: 10.1016/0304-4165(87)90017-1. [DOI] [PubMed] [Google Scholar]
- Usui T., Matsui H., Isobe K. Enzymic synthesis of useful chito-oligosaccharides utilizing transglycosylation by chitinolytic enzymes in a buffer containing ammonium sulfate. Carbohydr Res. 1990 Aug 1;203(1):65–77. doi: 10.1016/0008-6215(90)80046-6. [DOI] [PubMed] [Google Scholar]
- Watanabe T., Suzuki K., Oyanagi W., Ohnishi K., Tanaka H. Gene cloning of chitinase A1 from Bacillus circulans WL-12 revealed its evolutionary relationship to Serratia chitinase and to the type III homology units of fibronectin. J Biol Chem. 1990 Sep 15;265(26):15659–15665. [PubMed] [Google Scholar]