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. 1993 Feb;59(2):636–639. doi: 10.1128/aem.59.2.636-639.1993

Isolation of Rhizobium meliloti nod Gene Inducers from Alfalfa Rhizosphere Soil

Milagros León-Barrios 1,†, Felix D Dakora 1,‡, Cecillia M Joseph 1, Donald A Phillips 1,*
PMCID: PMC202160  PMID: 16348881

Abstract

Methanolic extracts of alfalfa rhizosphere soil induce more nod gene transcription in Rhizobium meliloti than extracts of nonrhizosphere soil. Six peaks of nod-inducing activity were separated by high-performance liquid chromatography from rhizosphere soil extract, and one compound was identified by 1H nuclear magnetic resonance, mass spectrometry, and UV-visible spectra as a formononetin-7-O-glycoside that activates both NodD1 and NodD2 proteins. The unanticipated presence of a glycoside in rhizosphere soil suggests either that large amounts of the glycoside were exuded by roots or that some glycosides are unexpectedly stable in soil.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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