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. 1993 Mar;59(3):772–776. doi: 10.1128/aem.59.3.772-776.1993

Antibody-magnetite method for selective concentration of Giardia lamblia cysts from water samples.

J M Bifulco 1, F W Schaefer 3rd 1
PMCID: PMC202188  PMID: 8481003

Abstract

An antibody-magnetite method was developed in order to selectively concentrate Giardia cysts from water samples. The indirect technique employed a mouse immunoglobulin G anti-Giardia antibody as a primary antibody and an anti-mouse immunoglobulin G antibody-coated magnetite particle as a secondary labeling reagent. The magnetically labeled cysts were then concentrated by high-gradient magnetic separation. Ninety percent of the seeded cysts were recovered from buffer when this method was employed. An average of 82% of the seeded cysts were recovered from water samples with various turbidities. Significantly higher cyst recoveries were obtained from water samples with turbidities below 600 nephelometric turbidity units.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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