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. 1993 Mar;59(3):915–918. doi: 10.1128/aem.59.3.915-918.1993

Direct extraction and purification of rRNA for ecological studies.

M A Moran 1, V L Torsvik 1, T Torsvik 1, R E Hodson 1
PMCID: PMC202208  PMID: 7683186

Abstract

Microgram quantities of rRNA were recovered from natural microbial communities in sediment, soil, and water with a lysozyme-hot phenol direct extraction method. Gel filtration with Sephadex G-75 spun columns readily removed humic-like contaminants without any measurable loss of rRNA and rendered RNA extracts of sufficient quality for molecular procedures.

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Selected References

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