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. 1993 Mar;59(3):936–938. doi: 10.1128/aem.59.3.936-938.1993

Comparison of fluorescent gentamicin-thallous-carbonate and KF streptococcal agars to enumerate enterococci and fecal streptococci in meats.

L M Knudtson 1, P A Hartman 1
PMCID: PMC202214  PMID: 8481014

Abstract

Two selective and differential media were compared for their abilities to enumerate enterococci and fecal streptococci in pork, beef, and poultry products. Counts obtained on KF streptococcal (KF) agar were compared with counts obtained on fluorescent gentamicin-thallous-carbonate (fGTC) agar. Reactions of 13 known enterococcal species were also observed. All 13 species of enterococci as well as Streptococcus bovis and Streptococcus equinus grew equally well on fGTC agar. KF streptococcal medium allowed growth of most species of enterococci but not S. bovis and S. equinus. Quantitative comparisons between the two media inoculated with pure cultures of known species of enterococci revealed equivalent plate counts following incubation. However, when meat samples were plated, counts on fGTC agar were consistently and significantly higher than counts on KF agar for all sample sources.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Donnelly L. S., Hartman P. A. Gentamicin-based medium for the isolation of group D streptococci and application of the medium to water analysis. Appl Environ Microbiol. 1978 Mar;35(3):576–581. doi: 10.1128/aem.35.3.576-581.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Hartman P. A., Reinbold G. W., Saraswat D. S. Media and methods for isolation and enumeration of the enterococci. Adv Appl Microbiol. 1966;8:253–289. [PubMed] [Google Scholar]
  3. KENNER B. A., CLARK H. F., KABLER P. W. Fecal Streptococci. I. Cultivation and enumeration of Streptococci in surface waters. Appl Microbiol. 1961 Jan;9:15–20. doi: 10.1128/am.9.1.15-20.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Knudtson L. M., Hartman P. A. Routine procedures for isolation and identification of enterococci and fecal streptococci. Appl Environ Microbiol. 1992 Sep;58(9):3027–3031. doi: 10.1128/aem.58.9.3027-3031.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Lachica R. V., Hartman P. A. Two improved media for isolating and enumerating enterococci in certain frozen foods. J Appl Bacteriol. 1968 Mar;31(1):151–156. doi: 10.1111/j.1365-2672.1968.tb00352.x. [DOI] [PubMed] [Google Scholar]
  6. Littel K. J., Hartman P. A. Fluorogenic selective and differential medium for isolation of fecal streptococci. Appl Environ Microbiol. 1983 Feb;45(2):622–627. doi: 10.1128/aem.45.2.622-627.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Murray B. E. The life and times of the Enterococcus. Clin Microbiol Rev. 1990 Jan;3(1):46–65. doi: 10.1128/cmr.3.1.46. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Thian T. S., Hartman P. A. Gentamicin-thallous-carbonate medium for isolation of fecal streptococci from foods. Appl Environ Microbiol. 1981 Mar;41(3):724–728. doi: 10.1128/aem.41.3.724-728.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]

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