Skip to main content
NCBI home page
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1993 Mar;59(3):939–941. doi: 10.1128/aem.59.3.939-941.1993

Chromosome Polymorphisms among Strains of Hansenula polymorpha (syn. Pichia angusta)

Laura Marri 1,*, Gian Maria Rossolini 1, Giuseppe Satta 2
PMCID: PMC202215  PMID: 16348902

Abstract

Contour-clamped homogeneous electrophoresis and an embedded-agarose method of sample preparation were combined to carry out an analysis of the chromosome sets of nine strains of Hansenula polymorpha (syn. Pichia angusta). Chromosomal DNA molecules could be separated into a series of bands ranging, approximately, from 650 up to 2,200 kb in size. Polymorphism of the electrophoretic pattern was demonstrated among the strains investigated in this study. Cross-hybridization between H. polymorpha and Saccharomyces cerevisiae ribosomal DNA was also observed.

Full text

PDF
939

Images in this article

940Image
on p.940
940Image
on p.940

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Burke D. T., Carle G. F., Olson M. V. Cloning of large segments of exogenous DNA into yeast by means of artificial chromosome vectors. Science. 1987 May 15;236(4803):806–812. doi: 10.1126/science.3033825. [DOI] [PubMed] [Google Scholar]
  2. Carle G. F., Olson M. V. An electrophoretic karyotype for yeast. Proc Natl Acad Sci U S A. 1985 Jun;82(11):3756–3760. doi: 10.1073/pnas.82.11.3756. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Carle G. F., Olson M. V. Orthogonal-field-alternation gel electrophoresis. Methods Enzymol. 1987;155:468–482. doi: 10.1016/0076-6879(87)55031-5. [DOI] [PubMed] [Google Scholar]
  4. Chu G., Vollrath D., Davis R. W. Separation of large DNA molecules by contour-clamped homogeneous electric fields. Science. 1986 Dec 19;234(4783):1582–1585. doi: 10.1126/science.3538420. [DOI] [PubMed] [Google Scholar]
  5. Feinberg A. P., Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983 Jul 1;132(1):6–13. doi: 10.1016/0003-2697(83)90418-9. [DOI] [PubMed] [Google Scholar]
  6. Gellissen G., Janowicz Z. A., Merckelbach A., Piontek M., Keup P., Weydemann U., Hollenberg C. P., Strasser A. W. Heterologous gene expression in Hansenula polymorpha: efficient secretion of glucoamylase. Biotechnology (N Y) 1991 Mar;9(3):291–295. doi: 10.1038/nbt0391-291. [DOI] [PubMed] [Google Scholar]
  7. Kurtzman C. P. Synonomy of the yeast genera Hansenula and Pichia demonstrated through comparisons of deoxyribonucleic acid relatedness. Antonie Van Leeuwenhoek. 1984;50(3):209–217. doi: 10.1007/BF02342132. [DOI] [PubMed] [Google Scholar]
  8. Mahrous M., Lott T. J., Meyer S. A., Sawant A. D., Ahearn D. G. Electrophoretic karyotyping of typical and atypical Candida albicans. J Clin Microbiol. 1990 May;28(5):876–881. doi: 10.1128/jcm.28.5.876-881.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Mortimer R. K., Schild D. Genetic map of Saccharomyces cerevisiae, edition 9. Microbiol Rev. 1985 Sep;49(3):181–213. doi: 10.1128/mr.49.3.181-213.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Orbach M. J., Vollrath D., Davis R. W., Yanofsky C. An electrophoretic karyotype of Neurospora crassa. Mol Cell Biol. 1988 Apr;8(4):1469–1473. doi: 10.1128/mcb.8.4.1469. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Perfect J. R., Magee B. B., Magee P. T. Separation of chromosomes of Cryptococcus neoformans by pulsed field gel electrophoresis. Infect Immun. 1989 Sep;57(9):2624–2627. doi: 10.1128/iai.57.9.2624-2627.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Polacheck I., Lebens G. A. Electrophoretic karyotype of the pathogenic yeast Cryptococcus neoformans. J Gen Microbiol. 1989 Jan;135(1):65–71. doi: 10.1099/00221287-135-1-65. [DOI] [PubMed] [Google Scholar]
  13. Rustchenko-Bulgac E. P. Variations of Candida albicans electrophoretic karyotypes. J Bacteriol. 1991 Oct;173(20):6586–6596. doi: 10.1128/jb.173.20.6586-6596.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Schwartz D. C., Cantor C. R. Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis. Cell. 1984 May;37(1):67–75. doi: 10.1016/0092-8674(84)90301-5. [DOI] [PubMed] [Google Scholar]
  15. Veldman G. M., Klootwijk J., de Regt V. C., Planta R. J., Branlant C., Krol A., Ebel J. P. The primary and secondary structure of yeast 26S rRNA. Nucleic Acids Res. 1981 Dec 21;9(24):6935–6952. doi: 10.1093/nar/9.24.6935. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. de Jonge P., de Jongh F. C., Meijers R., Steensma H. Y., Scheffers W. A. Orthogonal-field-alternation gel electrophoresis banding patterns of DNA from yeasts. Yeast. 1986 Sep;2(3):193–204. doi: 10.1002/yea.320020307. [DOI] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES