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. 1993 Apr;59(4):968–973. doi: 10.1128/aem.59.4.968-973.1993

Inhibitory effect of oolong tea polyphenols on glycosyltransferases of mutans Streptococci.

K Nakahara 1, S Kawabata 1, H Ono 1, K Ogura 1, T Tanaka 1, T Ooshima 1, S Hamada 1
PMCID: PMC202224  PMID: 8489234

Abstract

Oolong tea extract (OTE) was found to inhibit the water-insoluble glucan-synthesizing enzyme, glucosyltransferase I (GTase-I), of Streptococcus sobrinus 6715. The GTase-inhibitory substance in the OTE was purified successive adsorption chromatography on Diaion HP-21 and HP-20 columns; this was followed by further purification by Sephadex LH-20 column chromatography. A major fraction that inhibited GTase activity (fraction OTF10) was obtained, and the chemical analysis of OTF10 indicated that it was a novel polymeric polyphenol compound that had a molecular weight of approximately 2,000 and differed from other tea polyphenols. Catechins and all other low-molecular-weight polyphenols except theaflavin derived from balck tea did not show significant GTase-inhibitory activities. It was found that OTE amd PTF10 markedly inhibit GTase-I and yeast alpha-glucosidase, but not salivary alpha-amylase. Various GTases purified from S. sobrinus and Streptococcus mutans were examined for inhibition by OTE and OTF10. It was determined that S. sobrinus GTase-I and S. mutans cell-free GTase synthesizing water-soluble glucan were most susceptible to the inhibitory action of OTF10, while S. sobrinus GTase-Sa and S. mutans cell-associated GTase were moderately inhibited; no inhibition of S. sobrinus GTase-Sb was observed. Inhibition of a specific GTase or specific GTases of mutants streptococci resulted in decreased adherence of the growing cells of these organisms. The inhibitory effect of OTF10 on cellular adherence was significantly stronger than that of OTE.

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Selected References

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  1. Furuta T., Koga T., Nisizawa T., Okahashi N., Hamada S. Purification and characterization of glucosyltransferases from Streptococcus mutans 6715. J Gen Microbiol. 1985 Feb;131(2):285–293. doi: 10.1099/00221287-131-2-285. [DOI] [PubMed] [Google Scholar]
  2. Hamada S., Horikoshi T., Minami T., Kawabata S., Hiraoka J., Fujiwara T., Ooshima T. Oral passive immunization against dental caries in rats by use of hen egg yolk antibodies specific for cell-associated glucosyltransferase of Streptococcus mutans. Infect Immun. 1991 Nov;59(11):4161–4167. doi: 10.1128/iai.59.11.4161-4167.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hamada S., Horikoshi T., Minami T., Okahashi N., Koga T. Purification and characterization of cell-associated glucosyltransferase synthesizing water-insoluble glucan from serotype c Streptococcus mutans. J Gen Microbiol. 1989 Feb;135(Pt 2):335–344. doi: 10.1099/00221287-135-2-335. [DOI] [PubMed] [Google Scholar]
  4. Hamada S., Koga T., Ooshima T. Virulence factors of Streptococcus mutans and dental caries prevention. J Dent Res. 1984 Mar;63(3):407–411. doi: 10.1177/00220345840630031001. [DOI] [PubMed] [Google Scholar]
  5. Hamada S., Torii M. Effect of sucrose in culture media on the location of glucosyltransferase of Streptococcus mutans and cell adherence to glass surfaces. Infect Immun. 1978 Jun;20(3):592–599. doi: 10.1128/iai.20.3.592-599.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hattori M., Kusumoto I. T., Namba T., Ishigami T., Hara Y. Effect of tea polyphenols on glucan synthesis by glucosyltransferase from Streptococcus mutans. Chem Pharm Bull (Tokyo) 1990 Mar;38(3):717–720. doi: 10.1248/cpb.38.717. [DOI] [PubMed] [Google Scholar]
  7. Inoue M., Koga T., Sato S., Hamada S. Synthesis of adherent insoluble glucan by the concerted action of the two glucosyltransferase components of Streptococcus mutans. FEBS Lett. 1982 Jun 21;143(1):101–104. doi: 10.1016/0014-5793(82)80282-2. [DOI] [PubMed] [Google Scholar]
  8. Kakiuchi N., Hattori M., Nishizawa M., Yamagishi T., Okuda T., Namba T. Studies on dental caries prevention by traditional medicines. VIII. Inhibitory effect of various tannins on glucan synthesis by glucosyltransferase from Streptococcus mutans. Chem Pharm Bull (Tokyo) 1986 Feb;34(2):720–725. doi: 10.1248/cpb.34.720. [DOI] [PubMed] [Google Scholar]
  9. Kashket S., Paolino V. J., Lewis D. A., van Houte J. In-vitro inhibition of glucosyltransferase from the dental plaque bacterium Streptococcus mutans by common beverages and food extracts. Arch Oral Biol. 1985;30(11-12):821–826. doi: 10.1016/0003-9969(85)90138-4. [DOI] [PubMed] [Google Scholar]
  10. Koga T., Asakawa H., Okahashi N., Hamada S. Sucrose-dependent cell adherence and cariogenicity of serotype c Streptococcus mutans. J Gen Microbiol. 1986 Oct;132(10):2873–2883. doi: 10.1099/00221287-132-10-2873. [DOI] [PubMed] [Google Scholar]
  11. Koga T., Hamada S., Murakawa S., Endo A. Effect of a glucosyltransferase inhibitor on glucan synthesis and cellular adherence of Streptococcus mutans. Infect Immun. 1982 Dec;38(3):882–886. doi: 10.1128/iai.38.3.882-886.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Koga T., Horikoshi T., Fujiwara T., Hamada S. Effects of panose on glucan synthesis and cellular adherence by Streptococcus mutans. Microbiol Immunol. 1988;32(1):25–31. doi: 10.1111/j.1348-0421.1988.tb01362.x. [DOI] [PubMed] [Google Scholar]
  13. Minami T., Fujiwara T., Ooshima T., Nakajima Y., Hamada S. Interaction of structural isomers of sucrose in the reaction between sucrose and glucosyltransferases from mutans streptococci. Oral Microbiol Immunol. 1990 Aug;5(4):189–194. doi: 10.1111/j.1399-302x.1990.tb00644.x. [DOI] [PubMed] [Google Scholar]
  14. Onisi M., Ozaki F., Yoshino F., Murakami Y. An experimental evidence of caries preventive activity of non-fluoride component in tea. Koku Eisei Gakkai Zasshi. 1981 Jul;31(2):158–162. doi: 10.5834/jdh.31.158. [DOI] [PubMed] [Google Scholar]
  15. Ooshima T., Fujiwara T., Takei T., Izumitani A., Sobue S., Hamada S. The caries inhibitory effects of GOS-sugar in vitro and in rat experiments. Microbiol Immunol. 1988;32(11):1093–1105. doi: 10.1111/j.1348-0421.1988.tb01474.x. [DOI] [PubMed] [Google Scholar]
  16. Ooshima T., Izumitani A., Minami T., Fujiwara T., Nakajima Y., Hamada S. Trehalulose does not induce dental caries in rats infected with mutans streptococci. Caries Res. 1991;25(4):277–282. doi: 10.1159/000261376. [DOI] [PubMed] [Google Scholar]
  17. Ooshima T., Izumitani A., Minami T., Yoshida T., Sobue S., Fujiwara T., Hamada S. Noncariogenicity of maltitol in specific pathogen-free rats infected with mutans streptococci. Caries Res. 1992;26(1):33–37. doi: 10.1159/000261424. [DOI] [PubMed] [Google Scholar]
  18. Ooshima T., Izumitani A., Sobue S., Hamada S. Cariostatic effect of palatinose on experimental dental caries in rats. Jpn J Med Sci Biol. 1983 Aug;36(4):219–223. doi: 10.7883/yoken1952.36.219. [DOI] [PubMed] [Google Scholar]
  19. Ooshima T., Izumitani A., Sobue S., Okahashi N., Hamada S. Non-cariogenicity of the disaccharide palatinose in experimental dental caries of rats. Infect Immun. 1983 Jan;39(1):43–49. doi: 10.1128/iai.39.1.43-49.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Sela M. N., Steinberg D., Segal R. Inhibition of the activity of glucosyltransferase from Streptococcus mutans by glycyrrhizin. Oral Microbiol Immunol. 1987 Sep;2(3):125–128. doi: 10.1111/j.1399-302x.1987.tb00275.x. [DOI] [PubMed] [Google Scholar]

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