Abstract
The triacylglycerol (TG) profiles of teliospores of Tilletia controversa and Tilletia tritici were examined by high-performance liquid chromatography (HPLC) and gas-liquid chromatography. Boiling isopropanol was used to ensure enzyme inactivation during homogenization. The largest lipid component as determined by thin-layer chromatography was TGs. On the basis of thin-layer chromatography of crude lipid extracts, T. controversa and T. tritici do not contain a large amount of free fatty acids. TG profiles of T. controversa and T. tritici were very similar, with 18 species of TGs resolved by HPLC and gas-liquid chromatography. In both organisms, PLL (palmitic, linoleic, linoleic) was the major component, followed by LLL (trilinolein) and PLO (palmitic, linoleic, oleic). The ratio of PLO to PLL was 1:6 and 1:4 in T. tritici and T. controversa, respectively. The TGs of both organisms contain long-chain (>22 carbons) mono- and dienoic acids. Linoleic acid was the major fatty acid found in TGs from both organisms. The differences of TGs were not considered significant for differentiation purposes.
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Selected References
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- Gardner J. S., Hess W. M. Ultrastructure of lipid bodies in Tilletia caries teliospores. J Bacteriol. 1977 Aug;131(2):662–671. doi: 10.1128/jb.131.2.662-671.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Redshaw E. S., Zalik S. Changes in lipids of cereal seedlings during vernalization. Can J Biochem. 1968 Sep;46(9):1093–1097. doi: 10.1139/o68-163. [DOI] [PubMed] [Google Scholar]
- TULLOCH A. P., LEDINGHAM G. A. The component fatty acids of oils found in spores of plant rusts and other fungi. Can J Microbiol. 1960 Aug;6:425–434. doi: 10.1139/m60-048. [DOI] [PubMed] [Google Scholar]