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. 1993 Apr;59(4):1176–1180. doi: 10.1128/aem.59.4.1176-1180.1993

Isolation and Characterization of a Mo6+ -Reducing Bacterium

Baharuddin Ghani 1, Masataka Takai 2, N Zul Hisham 1, Noriaki Kishimoto 3, A K Mohamed Ismail 1, Tatsuo Tano 2, Tsuyoshi Sugio 2,*
PMCID: PMC202257  PMID: 16348915

Abstract

A Mo6+ -reducing bacterium (strain 48), which grew on medium supplemented with 200 mM Mo6+, was isolated from stream water obtained from Chengkau, Malaysia. The chemical properties of strain 48 conform to the characteristics of Enterobacter cloacae. Under anaerobic conditions in the glucose-yeast extract medium containing phosphate ion (2.9 mM) and Mo6+ (10 mM), the bacterium reduced Mo6+ to form molybdenum blue. Approximately 27% of Mo6+ added to the medium was reduced after 28 h of cultivation. The reduction of Mo6+ with glucose as an electron donor was strongly inhibited by iodoacetic acid, sodium fluoride, and sodium cyanide, suggesting an involvement of the glycolytic pathway and electron transport in Mo6+ reduction. NADH and N,N,N′,N′ -tetramethyl-p-phenylenediamine served as electron donors for Mo6+ reduction. When NADH was used as an electron donor, at first cytochrome b in the cell extract was reduced, and then molybdenum blue was formed. Sodium cyanide strongly inhibited Mo6+ reduction by NADH (5 mM) but not the reduction of cytochrome b in the cell extract, suggesting that the reduced component of the electron transport system after cytochrome b serves as an electron donor for Mo6+ reduction. Both ferric and stannous ions strongly enhanced the activity of Mo6+ reduction by NADH.

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Selected References

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