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. 1993 Apr;59(4):1253–1258. doi: 10.1128/aem.59.4.1253-1258.1993

Expression and secretion of Bacillus amyloliquefaciens alpha-amylase by using the yeast pheromone alpha-factor promoter and leader sequence in Saccharomyces cerevisiae.

V J Southgate 1, A J Steyn 1, I S Pretorius 1, H J Van Vuuren 1
PMCID: PMC202271  PMID: 8476297

Abstract

Replacement of the regulatory and secretory signals of the alpha-amylase gene (AMY) from Bacillus amylolique-faciens with the complete yeast pheromone alpha-factor prepro region (MF alpha 1p) resulted in increased levels of extracellular alpha-amylase production in Saccharomyces cerevisiae. However, the removal of the (Glu-Ala)2 peptide from the MF alpha 1 spacer region (Lys-Arg-Glu-Ala-Glu-Ala) yielded decreased levels of extracellular alpha-amylase.

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Selected References

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