Skip to main content
PMC home page
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1988 Feb;54(2):473–475. doi: 10.1128/aem.54.2.473-475.1988

Effects of Mannose on the Growth of N2-Fixing Azotobacter vinelandii

Tit-Yee Wong 1
PMCID: PMC202475  PMID: 16347561

Abstract

Mannose is not a suitable substrate for N2-fixing Azotobacter vinelandii. However, when H2 gas is provided, A. vinelandii can grow mixotrophically with H2 as the energy source and mannose as the carbon source (T.-Y. Wong and R. J. Maier, J. Bacteriol. 163:528-533, 1985). In this report, seven sugars were used to determine whether A. vinelandii could derive energy from these sugars for mannose utilization. Supplementation of fructose- or galactose-limited medium with mannose did not influence the biomass produced by N2-fixing A. vinelandii. The presence of mannose in glucose- or maltose-limited cultures increased cell yield slightly. The addition of mannose decreased the total biomass in the melibiose-limited culture slightly. Mannose was a potent inhibitor of growth when sucrose or turanose was used as the primary sugar. The inhibitory effect of mannose on utilization of sucrose and turanose seems to be related to the energy requirement of the N2-fixing processes.

Full text

PDF
473

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Jurtshuk P., Jr, Mueller T. J., Wong T. Y. Isolation and purification of the cytochrome oxidase of Azotobacter vinelandii. Biochim Biophys Acta. 1981 Sep 14;637(2):374–382. doi: 10.1016/0005-2728(81)90176-6. [DOI] [PubMed] [Google Scholar]
  2. MORTENSON L. E., HAMILTON P. B., WILSON P. W. Dissimilation of 6-phosphogluconate by Azotobacter vinelandii. Biochim Biophys Acta. 1955 Feb;16(2):238–244. doi: 10.1016/0006-3002(55)90209-2. [DOI] [PubMed] [Google Scholar]
  3. MORTENSON L. E., WILSON P. W. Initial stages in the breakdown of carbohydrates by the Azotobacter vinelandii. Arch Biochem Biophys. 1954 Dec;53(2):425–435. doi: 10.1016/0003-9861(54)90423-3. [DOI] [PubMed] [Google Scholar]
  4. Pindar D. F., Bucke C. The biosynthesis of alginic acid by Azotobacter vinelandii. Biochem J. 1975 Dec;152(3):617–622. doi: 10.1042/bj1520617. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Robson R. L., Postgate J. R. Oxygen and hydrogen in biological nitrogen fixation. Annu Rev Microbiol. 1980;34:183–207. doi: 10.1146/annurev.mi.34.100180.001151. [DOI] [PubMed] [Google Scholar]
  6. STILL G. G., WANG C. H. GLUCOSE CATABOLISM IN AZOTOBACTER VINELANDII. Arch Biochem Biophys. 1964 Apr;105:126–132. doi: 10.1016/0003-9861(64)90243-7. [DOI] [PubMed] [Google Scholar]
  7. Wong T. Y., Maier R. J. H2-dependent mixotrophic growth of N2-fixing Azotobacter vinelandii. J Bacteriol. 1985 Aug;163(2):528–533. doi: 10.1128/jb.163.2.528-533.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Wong T. Y., Maier R. J. Hydrogen-oxidizing electron transport components in nitrogen-fixing Azotobacter vinelandii. J Bacteriol. 1984 Jul;159(1):348–352. doi: 10.1128/jb.159.1.348-352.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES