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. 1975 Jul;32(1):51–59. doi: 10.1038/bjc.1975.133

Stimulation of granulocytic colony formation in agar diffusion chambers implanted in cyclophosphamide pretreated mice.

M Y Gordon, N M Blackett
PMCID: PMC2024783  PMID: 1080672

Abstract

The growth of mouse bone marrow colonies in agar diffusion chambers (ADCs) was evaluated using host mice injected with saline or with cyclophosphamide (CY) before chamber insertion. The mice pretreated with cyclophosphamide proved more effective hosts than control (saline pretreated) mice, indicating that cyclophosphamide causes the elaboration of a stimulating factor acting on colony precursor cells. Assays of the factor for colony stimulating activity against mouse bone marrow cells in agar culture in vitro suggest that potentiation may be due to a slight temporary increase in the level of colony stimulating factor (CSF) in the chamber environment, although a parallel increase was not detected in the serum. Stem cell recovery from the ADCs, measured by spleen colony formation, suggests that the stimulus may act by increasing differentiation at the level of the pluripotential stem cell.

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Selected References

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  1. Boyum A., Boecker W., Carsten A. L., Cronkite E. P. Proliferation of human bone marrow cells in diffusion chambers implanted into normal or irradiated mice. Blood. 1972 Aug;40(2):163–173. [PubMed] [Google Scholar]
  2. Bradley T. R., Fry P., Sumner M. A., McInerney E. Factors determining colony forming efficiency in agar suspension cultures. Aust J Exp Biol Med Sci. 1972 Dec;50(7):813–818. doi: 10.1038/icb.1972.78. [DOI] [PubMed] [Google Scholar]
  3. Bradley T. R., Metcalf D. The growth of mouse bone marrow cells in vitro. Aust J Exp Biol Med Sci. 1966 Jun;44(3):287–299. doi: 10.1038/icb.1966.28. [DOI] [PubMed] [Google Scholar]
  4. Bradley T. R., Metcalf D. The growth of mouse bone marrow cells in vitro. Aust J Exp Biol Med Sci. 1966 Jun;44(3):287–299. doi: 10.1038/icb.1966.28. [DOI] [PubMed] [Google Scholar]
  5. Burke P. J., Diggs C. H., Owens A. H., Jr Factors in human serum affecting the proliferation of normal and leukemic cells. Cancer Res. 1973 Apr;33(4):800–806. [PubMed] [Google Scholar]
  6. Fried W., Husseini S., Gregory S., Knospe W. H., Trobaugh F. E., Jr Effect of cyclophosphamide on the hematopoietic microenvironmental factors which influence hematopoietic stem cell proliferation. Cell Tissue Kinet. 1973 Mar;6(2):155–163. doi: 10.1111/j.1365-2184.1973.tb01604.x. [DOI] [PubMed] [Google Scholar]
  7. GORDON A. S., HANDLER E. S., SIEGEL C. D., DORNFEST B. S., LOBUE J. PLASMA FACTORS INFLUENCING LEUKOCYTE RELEASE IN RATS. Ann N Y Acad Sci. 1964 Feb 28;113:766–789. doi: 10.1111/j.1749-6632.1964.tb40703.x. [DOI] [PubMed] [Google Scholar]
  8. Gordon M. Y. Quantitation of haemopoietic cells from normal and leukaemic RFM mice using an in vivo colony assay. Br J Cancer. 1974 Nov;30(5):421–428. doi: 10.1038/bjc.1974.216. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gregory S. A., Fried W., Knospe W. H., Trobaugh F. E., Jr Accelerated regeneration of transplanted hematopoietic stem cells in irradiated mice pretreated with cyclophosphamide. Blood. 1971 Feb;37(2):196–203. [PubMed] [Google Scholar]
  10. Metcalf D., Stanley E. R. Haematological effects in mice of partially purified colony stimulating factor (CSF) prepared from human urine. Br J Haematol. 1971 Oct;21(4):481–492. doi: 10.1111/j.1365-2141.1971.tb02709.x. [DOI] [PubMed] [Google Scholar]
  11. Millard R. E., Blackett N. M., Okell S. F. A comparison of the effect of cytotoxic agents on agar colony forming cells, spleen colony forming cells, and the erythrocytic repopulating ability of mouse bone marrow. J Cell Physiol. 1973 Oct;82(2):309–317. doi: 10.1002/jcp.1040820219. [DOI] [PubMed] [Google Scholar]
  12. Morley A., Rickard K., Howard D., Stohlman F., Jr Studies on the regulation of granulopoiesis. IV. Possible humoral regulation. Blood. 1971 Jan;37(1):14–22. [PubMed] [Google Scholar]
  13. Pluznik D. H., Sachs L. The cloning of normal "mast" cells in tissue culture. J Cell Physiol. 1965 Dec;66(3):319–324. doi: 10.1002/jcp.1030660309. [DOI] [PubMed] [Google Scholar]
  14. Rencricca N. J., Rizzoli V., Howard D., Duffy P., Stohlman F., Jr Stem cell migration and proliferation during severe anemia. Blood. 1970 Dec;36(6):764–771. [PubMed] [Google Scholar]
  15. Rothstein G., Hügl E. H., Bishop C. R., Athens J. W., Ashenbrucker H. E. STimulation of granulocytopoiesis by a diffusible factor in vivo. J Clin Invest. 1971 Sep;50(9):2004–2007. doi: 10.1172/JCI106693. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Stanley E. R., Bradley T. R., Sumner M. A. Properties of the mouse embryo conditioned medium factor(s) stimulationg colony formation by mouse bone marrow cells grown in vitro. J Cell Physiol. 1971 Oct;78(2):301–317. doi: 10.1002/jcp.1040780219. [DOI] [PubMed] [Google Scholar]
  17. TILL J. E., McCULLOCH E. A. A direct measurement of the radiation sensitivity of normal mouse bone marrow cells. Radiat Res. 1961 Feb;14:213–222. [PubMed] [Google Scholar]
  18. Tyler W. S., Niskanen E., Stohlman F., Jr, Keane J., Howard D. The effect of neutropenia on myeloid growth and the stem cell in an in vivo culture system. Blood. 1972 Nov;40(5):634–645. [PubMed] [Google Scholar]

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