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. 1976 Feb;33(2):217–225. doi: 10.1038/bjc.1976.28

Studies on the relationship between concanavalin A and SV40-transformed human fibroblasts.

T Webb
PMCID: PMC2024949  PMID: 177037

Abstract

The extent of binding of 125I-Con A to the surface of SV40-transformed human fibroblasts and the degree of agglutination of the cells by the native lectin have been measured. In addition, trypsinized and succinylated Con A have been used to study the effects of the lectin upon certain cell growth parameters. Trypsinized Con A was found to alter the growth rate, the saturation density and the contact inhibition of the transformed cells, an effect not neutralized by alph-methyl-D-mannoside.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Burger M. M., Noonan K. D. Restoration of normal growth by covering of agglutinin sites on tumour cell surface. Nature. 1970 Nov 7;228(5271):512–515. doi: 10.1038/228512a0. [DOI] [PubMed] [Google Scholar]
  2. Cline M. J., Livingston D. C. Binding of 3 H-concanavalin A by normal and transformed cells. Nat New Biol. 1971 Aug 4;232(31):155–156. doi: 10.1038/newbio232155a0. [DOI] [PubMed] [Google Scholar]
  3. Cunningham B. A., Wang J. L., Pflumm M. N., Edelman G. M. Isolation and proteolytic cleavage of the intact subunit of concanavalin A. Biochemistry. 1972 Aug 15;11(17):3233–3239. doi: 10.1021/bi00767a016. [DOI] [PubMed] [Google Scholar]
  4. De Salle L., Munakata N., Pauli R. M., Strauss B. S. Receptor sites for concanavalin A on human peripheral lymphocytes and on lymphoblasts grown in long-term culture. Cancer Res. 1972 Nov;32(11):2463–2468. [PubMed] [Google Scholar]
  5. Gunther G. R., Wang J. L., Yahara I., Cunningham B. A., Edelman G. M. Concanavalin A derivatives with altered biological activities. Proc Natl Acad Sci U S A. 1973 Apr;70(4):1012–1016. doi: 10.1073/pnas.70.4.1012. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. HUNTER W. M., GREENWOOD F. C. Preparation of iodine-131 labelled human growth hormone of high specific activity. Nature. 1962 May 5;194:495–496. doi: 10.1038/194495a0. [DOI] [PubMed] [Google Scholar]
  7. Hakomori S. Cell density-dependent changes of glycolipid concentrations in fibroblasts, and loss of this response in virus-transformed cells. Proc Natl Acad Sci U S A. 1970 Dec;67(4):1741–1747. doi: 10.1073/pnas.67.4.1741. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Inbar M., Ben-Bassat H., Sachs L. Inhibition of ascites tumor development by concanavalin A. Int J Cancer. 1972 Jan 15;9(1):143–149. doi: 10.1002/ijc.2910090117. [DOI] [PubMed] [Google Scholar]
  9. Inbar M., Ben-Bassat H., Sachs L. Membrane changes associated with malignancy. Nat New Biol. 1972 Mar 1;236(61):3–passim. doi: 10.1038/newbio236003a0. [DOI] [PubMed] [Google Scholar]
  10. Inbar M., Sachs L. Interaction of the carbohydrate-binding protein concanavalin A with normal and transformed cells. Proc Natl Acad Sci U S A. 1969 Aug;63(4):1418–1425. doi: 10.1073/pnas.63.4.1418. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kao F. T., Harris H. Lack of correlation between malignancy and sensitivity to killing by concanavalin A. J Natl Cancer Inst. 1975 Mar;54(3):767–768. [PubMed] [Google Scholar]
  12. Moore E. G., Temin H. M. Lack of correlation between conversion of RNA tumour viruses and increased agglutinability of cells by concanavalin A and wheat germ agglutinin. Nature. 1971 May 14;231(5298):117–118. doi: 10.1038/231117a0. [DOI] [PubMed] [Google Scholar]
  13. Moscona A. A. Embryonic and neoplastic cell surfaces: availability of receptors for concanavalin A and wheat germ agglutinin. Science. 1971 Mar 5;171(3974):905–907. doi: 10.1126/science.171.3974.905. [DOI] [PubMed] [Google Scholar]
  14. Nicolson G. L. Temperature-dependent mobility of concanavalin A sites on tumour cell surfaces. Nat New Biol. 1973 Jun 13;243(128):218–220. doi: 10.1038/newbio243218a0. [DOI] [PubMed] [Google Scholar]
  15. Nicolson G. L. Topography of membrane concanavalin A sites modified by proteolysis. Nat New Biol. 1972 Oct 18;239(94):193–197. doi: 10.1038/newbio239193a0. [DOI] [PubMed] [Google Scholar]
  16. Ozanne B., Sambrook J. Binding of radioactively labelled concanavalin A and wheat germ agglutinin to normal and virus-transformed cells. Nat New Biol. 1971 Aug 4;232(31):156–160. doi: 10.1038/newbio232156a0. [DOI] [PubMed] [Google Scholar]
  17. Ozanne B. Variants of simian virus 40-transformed 3T3 cells that are resistant to concanavalin A. J Virol. 1973 Jul;12(1):79–89. doi: 10.1128/jvi.12.1.79-89.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Pollack R. E., Burger M. M. Surface-specific characteristics of a contact-inhibited cell line containing the SV40 viral genome. Proc Natl Acad Sci U S A. 1969 Apr;62(4):1074–1076. doi: 10.1073/pnas.62.4.1074. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Rowlatt C., Wicker R., Bernhard W. Ultrastructural distribution of concanavalin-A receptors on hamster embryo and adenovirus tumour cell cultures. Int J Cancer. 1973 Mar 15;11(2):314–326. doi: 10.1002/ijc.2910110209. [DOI] [PubMed] [Google Scholar]
  20. Shoham J., Sachs L. Differences in the binding of fluorescent concanavalin A to the surface membrane of normal and transformed cells. Proc Natl Acad Sci U S A. 1972 Sep;69(9):2479–2482. doi: 10.1073/pnas.69.9.2479. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Singer S. J., Nicolson G. L. The fluid mosaic model of the structure of cell membranes. Science. 1972 Feb 18;175(4023):720–731. doi: 10.1126/science.175.4023.720. [DOI] [PubMed] [Google Scholar]
  22. Smith H. S., Hiller A. J., Kingsbury E. W., Roberts-Dory C. Cell surface properties and the expression of SV40-induced transformation. Nat New Biol. 1973 Sep 19;245(142):67–69. doi: 10.1038/newbio245067a0. [DOI] [PubMed] [Google Scholar]
  23. Todaro G. J., Green H., Swift M. R. Susceptibility of human diploid fibroblast strains to transformation by SV40 virus. Science. 1966 Sep 9;153(3741):1252–1254. doi: 10.1126/science.153.3741.1252. [DOI] [PubMed] [Google Scholar]
  24. Trowbridge I. S., Hilborn D. A. Effects of succinyl-con A on the growth of normal and transformed cells. Nature. 1974 Jul 26;250(464):304–307. doi: 10.1038/250304a0. [DOI] [PubMed] [Google Scholar]

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