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. 1988 Feb;54(2):583–584. doi: 10.1128/aem.54.2.583-584.1988

Preparation of a Cell-Free, Isobutene-Forming System from Rhodotorula minuta

Takao Fujii 1,*, Takahira Ogawa 1, Hideo Fukuda 1
PMCID: PMC202497  PMID: 16347568

Abstract

The cell-free isobutene-forming system of Rhodotorula minuta IFO 1102 was prepared. This system consisted of isovaleric acid, NADPH, and a cell extract. The maximal activity obtained by using the described cell-free system was 17 nl/mg of protein per h. It appears that this cell-free isobutene-forming system operates in living cells of R. minuta.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]

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