Skip to main content
NCBI home page
British Journal of Cancer logoLink to British Journal of Cancer
. 1976 Mar;33(3):279–289. doi: 10.1038/bjc.1976.41

Characterization of cytotoxic spleen cells and effects of serum factors in a syngeneic rat tumour system.

N Matthews, P J Chalmers, G R Flannery, R C Nairn
PMCID: PMC2024971  PMID: 773397

Abstract

Splenocytes from inbred Wistar rats bearing a syngeneic squamous cell carcinoma (Spl) were fractionated by several techniques to characterize the lymphoid cells cytotoxic to the tumour in vitro. The anti-tumour cytotoxicity is presumably mediated primarily by T lymphocytes because it was greatly reduced by removal of T lymphocytes with heterologous anti-T serum plus complement but not by removal of other cell types. Cytotoxicity could be blocked at the tumour cell but not at the effector cell by sera taken late in tumour growth. Sera taken earlier in tumour growth could induce cytolysis of tumour cells by normal splenocytes but only if the tumour cells were treated with serum and washed before addition of the effector cells. Although splenocytes from normal and tumour-bearing rats were equally effective at lysing antibody-coated target cells it is unlikely that this mechanism is important in vivo as sera from early in tumour growth onwards contained factors (immune complexes?) which inhibited antibody-induced lymphocytolysis.

Full text

PDF
279

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baldwin R. W., Price M. R., Robins R. A. Blocking of lymphocyte-mediated cytotoxicity for rat hepatoma cells by tumour-specific antigen-antibody complexes. Nat New Biol. 1972 Aug 9;238(84):185–186. doi: 10.1038/newbio238185a0. [DOI] [PubMed] [Google Scholar]
  2. Baldwin R. W., Price M. R., Robins R. A. Inhibition of hepatoma-immune lymph-node cell cytotoxicity by tumour-bearer serum, and solubilized hepatoma antigen. Int J Cancer. 1973 May;11(3):527–535. doi: 10.1002/ijc.2910110304. [DOI] [PubMed] [Google Scholar]
  3. Baldwin R. W. Tumour-specific immunity against spontaneous rat tumours. Int J Cancer. 1966 May 15;1(3):257–264. doi: 10.1002/ijc.2910010305. [DOI] [PubMed] [Google Scholar]
  4. Basham C., Currie G. A. Development of specific cell-dependent antibody during growth of a syngeneic rat sarcoma. Br J Cancer. 1974 Mar;29(3):189–198. doi: 10.1038/bjc.1974.58. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bonavida B. Studies on the induction and expression of T cell-mediated immunity. II. Antiserum blocking of cell-mediated cytolysis. J Immunol. 1974 Apr;112(4):1308–1321. [PubMed] [Google Scholar]
  6. Cantor H. The effects of anti-theta antiserum upon graft-versus-host activity of spleen and lymph node cells. Cell Immunol. 1972 Mar;3(3):461–469. doi: 10.1016/0008-8749(72)90251-1. [DOI] [PubMed] [Google Scholar]
  7. Currie G. A., Basham C. Serum mediated inhibition of the immunological reactions of the patient to his own tumour: a possible role for circulating antigen. Br J Cancer. 1972 Dec;26(6):427–438. doi: 10.1038/bjc.1972.59. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Flannery G. R., Chalmers P. J., Rolland J. M., Nairn R. C. Immune response to a syngeneic rat tumour: development of regional node lymphocyte anergy. Br J Cancer. 1973 Aug;28(2):118–122. doi: 10.1038/bjc.1973.129. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Flannery G. R., Chalmers P. J., Rolland J. M., Nairn R. C. Immune response to a syngeneic rat tumour: evolution of serum cytotoxicity and blockade. Br J Cancer. 1973 Oct;28(4):293–298. doi: 10.1038/bjc.1973.151. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Flannery G. R., Muller H. K., Nairn R. C. Lymphoreticular response to a syngeneic rat tumour: gravimetric and histological studies. Br J Cancer. 1975 Jun;31(6):614–619. doi: 10.1038/bjc.1975.107. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Ford W. L., Burr W., Simonsen M. A lymph node weight assay for the graft-versus-host activity of rat lymphoid cells. Transplantation. 1970 Sep;10(3):258–266. doi: 10.1097/00007890-197009000-00007. [DOI] [PubMed] [Google Scholar]
  12. Gorczynski R. M., Kilburn D. G., Knight R. A., Norbury C., Parker D. C., Smith J. B. Nonspecific and specific immunosuppression in tumour-bearing mice by soluble immune complexes. Nature. 1975 Mar 13;254(5496):141–143. doi: 10.1038/254141a0. [DOI] [PubMed] [Google Scholar]
  13. Hellström I., Hellström K. E. Studies on cellular immunity and its serum mediated inhibition in Moloney-virus-induced mouse sarcomas. Int J Cancer. 1969 Sep 15;4(5):587–600. doi: 10.1002/ijc.2910040503. [DOI] [PubMed] [Google Scholar]
  14. Kirchner H., Chused T. M., Herberman R. B., Holden H. T., Lavrin D. H. Evidence of suppressor cell activity in spleens of mice bearing primary tumors induced by Moloney sarcoma virus. J Exp Med. 1974 Jun 1;139(6):1473–1487. doi: 10.1084/jem.139.6.1473. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Lamon E. W., Wigzel H., Klein E., Andersson B., Skurzak H. M. The lymphocyte response to primary Moloney sarcoma virus tumors in BALB-c mice. Definition of the active subpopulations at different times after infection. J Exp Med. 1973 Jun 1;137(6):1472–1493. doi: 10.1084/jem.137.6.1472. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Leclerc J. C., Senik A., Gomard E., Plata F., Levy J. P. Cell-mediated antitumor immune reactions under syngeneic conditions. Transplant Proc. 1973 Dec;5(4):1431–1434. [PubMed] [Google Scholar]
  17. MacLennan I. C. Competition for receptors for immunoglobulin on cytotoxic lymphocytes. Clin Exp Immunol. 1972 Feb;10(2):275–283. [PMC free article] [PubMed] [Google Scholar]
  18. Matthews N., Maclaurin B. P. "Spontaneous" cytolysis by normal human lymphocytes of Burkitt's lymphoma cells of the EB2 cell line. Aust J Exp Biol Med Sci. 1974 Aug;52(4):655–661. doi: 10.1038/icb.1974.64. [DOI] [PubMed] [Google Scholar]
  19. Matthews N., Rolland J. M., Nairn R. C. Lymphoid cell fractionation by aggregated immunoglobulin-agarose columns. J Immunol Methods. 1976;9(3-4):323–335. doi: 10.1016/0022-1759(76)90207-6. [DOI] [PubMed] [Google Scholar]
  20. Nind A. P., Matthews N., Pihl E. A., Rolland J. M., Nairn R. C. Analysis of inhibition of lymphocyte cytotoxicity in human colon carcinoma. Br J Cancer. 1975 Jun;31(6):620–629. doi: 10.1038/bjc.1975.108. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. O'Toole C., Stejskal V., Perlmann P., Karlsson M. Lymphoid cells mediating tumor-specific cytotoxicity to carcinoma of the urinary bladder. Separation of the effector population using a surface marker. J Exp Med. 1974 Mar 1;139(3):457–466. doi: 10.1084/jem.139.3.457. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Pollack S., Heppner G., Brawn R. J., Nelson K. Specific killing of tumor cells in vitro in the presence of normal lymphoid cells and sera from hosts immune to the tumor antigens. Int J Cancer. 1972 Mar 15;9(2):316–323. doi: 10.1002/ijc.2910090209. [DOI] [PubMed] [Google Scholar]
  23. Shellam G. R. Studies on a gross-virus-induced lymphoma in the rat. I. The cell-mediated immune response. Int J Cancer. 1974 Jul 15;14(1):65–82. doi: 10.1002/ijc.2910140109. [DOI] [PubMed] [Google Scholar]
  24. Takasugi M., Klein E. A microassay for cell-mediated immunity. Transplantation. 1970 Mar;9(3):219–227. doi: 10.1097/00007890-197003000-00005. [DOI] [PubMed] [Google Scholar]
  25. Todd R. F., 3rd, Stulting R. D., Berke G. Mechanism of blocking by hyperimmune serum of lymphocyte-mediated cytolysis of allogeneic tumor cells. Cancer Res. 1973 Dec;33(12):3203–3208. [PubMed] [Google Scholar]
  26. Wybran J., Hellström I., Hellström K. E., Fudenberg H. H. Cytotoxicity of human rosette-forming blood lymphocytes on cultivated human tumor cells. Int J Cancer. 1974 Apr 15;13(4):515–521. doi: 10.1002/ijc.2910130411. [DOI] [PubMed] [Google Scholar]
  27. van Dalen J. P., Knapp W., Ploem J. S. Microfluorometry on antigen-antibody interaction in immunofluorescence using antigens covalently bound to agarose beads. J Immunol Methods. 1973 Jun;2(4):383–392. doi: 10.1016/0022-1759(73)90004-5. [DOI] [PubMed] [Google Scholar]

Articles from British Journal of Cancer are provided here courtesy of Cancer Research UK

RESOURCES