Abstract
Different radioactive precursors were added to 8-day potato-dextrose liquid cultures of Aspergillus terreus 23-1. Territrems were isolated from chloroform extracts of the cultures at day 14 and purified by thin-layer chromatography and high-pressure liquid chromatography. The territrem B obtained was treated with alkaline hydrogen peroxide, and 3, 4, 5-trimethoxy benzoic acid was isolated from an ethyl acetate extract of the reaction mixture and purified by thin-layer chromatography and high-pressure liquid chromatography. By comparison of the specific radioactivities of territrem B and its cleaved aromatic product (disintegrations per minute per micromole of compound), it was demonstrated that the radioactivity of territrem B was located mainly on its aromatic moiety when [U-14C]shikimate, l-[methyl-14C]methionine, and l-[methyl-3H]methionine were precursors; however, the radioactivity of territrem B was located mainly on its nonaromatic moiety when [2-14C]mevalonate was the precursor. Mevinolin, a specific inhibitor of β-hydroxyl β-methyl glutaryl coenzyme A reductase, was shown to inhibit production of territrems by A. terreus 23-1. When [U-14C]acetate was used as a precursor, mevinolin inhibited the incorporation of radioactive carbon into territrem but mevinolin did not inhibit incorporation of radioactive carbon from [2-14C]mevalonate into territrem.
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- Alberts A. W., Chen J., Kuron G., Hunt V., Huff J., Hoffman C., Rothrock J., Lopez M., Joshua H., Harris E. Mevinolin: a highly potent competitive inhibitor of hydroxymethylglutaryl-coenzyme A reductase and a cholesterol-lowering agent. Proc Natl Acad Sci U S A. 1980 Jul;77(7):3957–3961. doi: 10.1073/pnas.77.7.3957. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bach T. J., Lichtenthaler H. K. Mechanisms of inhibition by mevinolin (MK 803) of microsome-bound radish and of partially purified yeast HMG-CoA reductase (EC.1.1.1.34). Z Naturforsch C. 1983 Mar-Apr;38(3-4):212–219. doi: 10.1515/znc-1983-3-410. [DOI] [PubMed] [Google Scholar]
- Brown A. G., Smale T. C., King T. J., Hasenkamp R., Thompson R. H. Crystal and molecular structure of compactin, a new antifungal metabolite from Penicillium brevicompactum. J Chem Soc Perkin 1. 1976;(11):1165–1170. [PubMed] [Google Scholar]
- Endo A., Kuroda M., Tsujita Y. ML-236A, ML-236B, and ML-236C, new inhibitors of cholesterogenesis produced by Penicillium citrinium. J Antibiot (Tokyo) 1976 Dec;29(12):1346–1348. doi: 10.7164/antibiotics.29.1346. [DOI] [PubMed] [Google Scholar]
- Ling K. H., Yang C. K., Kuo C. A., Kuo M. D. Solvent systems for improved isolation and separation of territrems A and B. Appl Environ Microbiol. 1982 Oct;44(4):860–863. doi: 10.1128/aem.44.4.860-863.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ling K. H., Yang C. K., Peng F. T. Territrems, tremorgenic mycotoxins of Aspergillus terreus. Appl Environ Microbiol. 1979 Mar;37(3):355–357. doi: 10.1128/aem.37.3.355-357.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]