Skip to main content
NCBI home page
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1988 Feb;54(2):596–599. doi: 10.1128/aem.54.2.596-599.1988

Differentiation of ruminal bacterial species by enzyme-linked immunosorbent assay using egg yolk antibodies from immunized chicken hens.

S C Ricke 1, D M Schaefer 1, M E Cook 1, K H Kang 1
PMCID: PMC202502  PMID: 3355145

Abstract

Cross-reactivity among four species of ruminal bacteria was examined by using egg yolk antibodies from immunized Leghorn laying hens and an enzyme-linked-immunosorbent assay. The effects of the four species on the hens were compared on various days postimmunization. Hens injected with the same bacterial species had similar apparent antibody levels over the entire postimmunization period, but only Bacteroides ruminicola B1(4) and Selenomonas ruminantium D antigens elicited early increases in apparent antibody levels during weeks 2 and 3. Antibody cross-reactivity was greatly reduced by week 2, except for antibodies against Streptococcus bovis JB1.

Full text

PDF
596

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bar-Joseph M., Malkinson M. Hen egg yolk as a source of antiviral antibodies in the enzyme-linked immunosorbent assay (ELISA): a comparison of two plant viruses. J Virol Methods. 1980;1(3):179–183. doi: 10.1016/0166-0934(80)90014-2. [DOI] [PubMed] [Google Scholar]
  2. Cheng K. J., Hironaka R., Jones G. A., Nicas T., Costerton J. W. Frothy feedlot bloat in cattle: production of extracellular polysaccharides and development of viscosity in cultures of Streptococcus bovis. Can J Microbiol. 1976 Apr;22(4):450–459. doi: 10.1139/m76-071. [DOI] [PubMed] [Google Scholar]
  3. Conway de Macario E., Macario A. J., Wolin M. J. Specific antisera and immunological procedures for characterization of methanogenic bacteria. J Bacteriol. 1982 Jan;149(1):320–328. doi: 10.1128/jb.149.1.320-328.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Faith R. E., Clem L. W. Passive cutaneous anaphylaxis in the chicken. Biological fractionation of the mediating antibody population. Immunology. 1973 Jul;25(1):151–164. [PMC free article] [PubMed] [Google Scholar]
  5. HOBSON P. N., MANN S. O., SMITH W. Serological tests of a relationship between rumen selenomonads in vitro and in vivo. J Gen Microbiol. 1962 Oct;29:265–270. doi: 10.1099/00221287-29-2-265. [DOI] [PubMed] [Google Scholar]
  6. Isaacson H. R., Hinds F. C., Bryant M. P., Owens F. N. Efficiency of energy utilization by mixed rumen bacteria in continuous culture. J Dairy Sci. 1975 Nov;58(11):1645–1659. doi: 10.3168/jds.S0022-0302(75)84763-1. [DOI] [PubMed] [Google Scholar]
  7. Kingsley V. V., Hoeniger J. F. Growth, structure, and classification of Selenomonas. Bacteriol Rev. 1973 Dec;37(4):479–521. doi: 10.1128/br.37.4.479-521.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  9. MARGHERITA S. S., HUNGATE R. E. SEROLOGICAL ANALYSIS OF BUTYRIVIBRIO FROM THE BOVINE RUMEN. J Bacteriol. 1963 Oct;86:855–860. doi: 10.1128/jb.86.4.855-860.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. MARGHERITA S. S., HUNGATE R. E., STORZ H. VARIATION IN RUMEN BUTYRIVIBRIO STRAINS. J Bacteriol. 1964 Jun;87:1304–1308. doi: 10.1128/jb.87.6.1304-1308.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Malkinson M. The transmission of passive immunity to Escherichia coli from mother to young in the domestic fowl (Gallus domesticus). Immunology. 1965 Oct;9(4):311–317. [PMC free article] [PubMed] [Google Scholar]
  12. Orlans E. Fowl antibody. 8. A comparison of natural, primary and secondary antibodies to erythrocytes in hen sera; their transmission to yolk and chick. Immunology. 1967 Jan;12(1):27–37. [PMC free article] [PubMed] [Google Scholar]
  13. PATTERSON R., YOUNGNER J. S., WEIGLE W. O., DIXON F. J. Antibody production and transfer to egg yolk in chickens. J Immunol. 1962 Aug;89:272–278. [PubMed] [Google Scholar]
  14. PATTERSON R., YOUNGNER J. S., WEIGLE W. O., DIXON F. J. The metabolism of serum proteins in the hen and chick and secretion of serum proteins by the ovary of the hen. J Gen Physiol. 1962 Jan;45:501–513. doi: 10.1085/jgp.45.3.501. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Polson A., von Wechmar M. B., van Regenmortel M. H. Isolation of viral IgY antibodies from yolks of immunized hens. Immunol Commun. 1980;9(5):475–493. doi: 10.3109/08820138009066010. [DOI] [PubMed] [Google Scholar]
  16. Poxton I. R., Brown R., Collee J. G. Detection of species-specific and cross-reactive cell-surface antigens of Bacteroides species by an indirect enzyme-linked immunosorbent assay. J Med Microbiol. 1982 May;15(2):223–231. doi: 10.1099/00222615-15-2-223. [DOI] [PubMed] [Google Scholar]
  17. Rose M. E., Orlans E. Immunoglobulins in the egg, embryo and young chick. Dev Comp Immunol. 1981 Winter;5(1):15–20. doi: 10.1016/s0145-305x(81)80003-1. [DOI] [PubMed] [Google Scholar]
  18. Schaefer D. M., Davis C. L., Bryant M. P. Ammonia saturation constants for predominant species of rumen bacteria. J Dairy Sci. 1980 Aug;63(8):1248–1263. doi: 10.3168/jds.S0022-0302(80)83076-1. [DOI] [PubMed] [Google Scholar]
  19. Sharpe M. E. Serology of rumen bacteroides. J Gen Microbiol. 1971 Aug;67(3):273–288. doi: 10.1099/00221287-67-3-273. [DOI] [PubMed] [Google Scholar]
  20. Slaght S. S., Yang T. J., van der Heide L., Fredrickson T. N. An enzyme-linked immunosorbent assay (ELISA) for detecting chicken anti-reovirus antibody at high sensitivity. Avian Dis. 1978 Oct-Dec;22(4):802–805. [PubMed] [Google Scholar]
  21. Teather R. M. Maintenance of Laboratory strains of obligately anaerobic rumen bacteria. Appl Environ Microbiol. 1982 Aug;44(2):499–501. doi: 10.1128/aem.44.2.499-501.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES