Skip to main content
PMC home page
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1988 Feb;54(2):617–618. doi: 10.1128/aem.54.2.617-618.1988

Evaluation of four methods for enumeration of Vibrio parahaemolyticus.

A DePaola 1, L H Hopkins 1, R M McPhearson 1
PMCID: PMC202508  PMID: 3281584

Abstract

Two membrane filter (MF) and two most-probable-number methods for enumerating Vibrio parahaemolyticus were compared. The MF methods used elevated-temperature incubations (41 and 42 degrees C) and were more specific than the most-probable-number methods (conducted at 35 degrees C). The MF method with a hydrophobic grid and a repair step was most effective.

Full text

PDF
617

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baross J., Liston J. Occurrence of Vibrio parahaemolyticus and related hemolytic vibrios in marine environments of Washington State. Appl Microbiol. 1970 Aug;20(2):179–186. doi: 10.1128/am.20.2.179-186.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bartley C. H., Slanetz L. W. Occurrence of Vibrio parahaemolyticus in estuarine waters and oysters of New Hampshire. Appl Microbiol. 1971 May;21(5):965–966. doi: 10.1128/am.21.5.965-966.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. DePaola A., Kaysner C. A., McPhearson R. M. Elevated temperature method for recovery of Vibrio cholerae from oysters (Crassostrea gigas). Appl Environ Microbiol. 1987 May;53(5):1181–1182. doi: 10.1128/aem.53.5.1181-1182.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. El-Sahn M. A., El-Banna A. A., El-Tabey Shehata A. M. Occurrence of Vibrio parahaemolyticus in selected marine invertebrates, sediment, and seawater around Alexandria, Egypt. Can J Microbiol. 1982 Nov;28(11):1261–1264. doi: 10.1139/m82-187. [DOI] [PubMed] [Google Scholar]
  5. Kampelmacher E. H., Mossel D. A., Van Noorle Jansen L. M., Vincentie H. A survey on the occurrence of Vibrio parahaemolyticus on fish and shellfish, marketed in the Netherlands. J Hyg (Lond) 1970 Jun;68(2):189–196. doi: 10.1017/s0022172400028667. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Ray B., Hawkins S. M., Hackney C. R. Method for the detection of injured Vibrio parahaemolyticus in seafoods. Appl Environ Microbiol. 1978 Jun;35(6):1121–1127. doi: 10.1128/aem.35.6.1121-1127.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Watkins W. D., Thomas C. D., Cabelli V. J. Membrane filter procedure for enumeration of Vibrio parahaemolyticus. Appl Environ Microbiol. 1976 Nov;32(5):679–684. doi: 10.1128/aem.32.5.679-684.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES