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. 1988 Mar;54(3):625–628. doi: 10.1128/aem.54.3.625-628.1988

Nutritional requirements of Methanomicrobium mobile.

R S Tanner 1, R S Wolfe 1
PMCID: PMC202515  PMID: 3377488

Abstract

A defined medium was developed for Methanomicrobium mobile BP. M. mobile required acetate for growth; the optimal concentration was 30 mM. Other requirements and their optimal concentrations included isobutyrate (0.65 mM), isovalerate (0.73 mM), and 2-methylbutyrate (1.5 mM). The appropriate branched-chain amino acids did not substitute for these branched-chain fatty acids. M. mobile required tryptophan at an optimal concentration of 24 microM. Indole substituted for tryptophan, but the possible precursor compounds shikimic acid and anthranilic acid and the degradation compound skatole did not. Vitamin requirements and their optimal concentrations included pyridoxine (0.49 microM), thiamine (0.15 microM), biotin (0.04 microM), and vitamin B12 (0.04 microM); p-aminobenzoic acid (0.18 microM) was required for optimal growth, but folic acid did not replace p-aminobenzoic acid. M. mobile required an unidentified growth factor found in ruminal fluid or extracts of Methanobacterium thermoautotrophicum for growth. M. mobile has a complex nutrition compared with that of other methanogens, but not an unusual nutrition in the context of organisms from the ruminal ecosystem.

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Selected References

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  1. Allison M. J. Production of branched-chain volatile fatty acids by certain anaerobic bacteria. Appl Environ Microbiol. 1978 May;35(5):872–877. doi: 10.1128/aem.35.5.872-877.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BRYANT M. P., ROBINSON I. M. Some nutritional characteristics of predominant culturable ruminal bacteria. J Bacteriol. 1962 Oct;84:605–614. doi: 10.1128/jb.84.4.605-614.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Balch W. E., Fox G. E., Magrum L. J., Woese C. R., Wolfe R. S. Methanogens: reevaluation of a unique biological group. Microbiol Rev. 1979 Jun;43(2):260–296. doi: 10.1128/mr.43.2.260-296.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Balch W. E., Wolfe R. S. New approach to the cultivation of methanogenic bacteria: 2-mercaptoethanesulfonic acid (HS-CoM)-dependent growth of Methanobacterium ruminantium in a pressureized atmosphere. Appl Environ Microbiol. 1976 Dec;32(6):781–791. doi: 10.1128/aem.32.6.781-791.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bryant M. P. Nutritional requirements of the predominant rumen cellulolytic bacteria. Fed Proc. 1973 Jul;32(7):1809–1813. [PubMed] [Google Scholar]
  6. Bryant M. P., Robinson I. M. Some Nutritional Requirements of the Genus Ruminococcus. Appl Microbiol. 1961 Mar;9(2):91–95. doi: 10.1128/am.9.2.91-95.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Conway de Macario E., Macario A. J., Wolin M. J. Antigenic analysis of Methanomicrobiales and Methanobrevibacter arboriphilus. J Bacteriol. 1982 Nov;152(2):762–764. doi: 10.1128/jb.152.2.762-764.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Jarrell K. F., Colvin J. R., Sprott G. D. Spontaneous protoplast formation in Methanobacterium bryantii. J Bacteriol. 1982 Jan;149(1):346–353. doi: 10.1128/jb.149.1.346-353.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Leigh J. A. Levels of water-soluble vitamins in methanogenic and non-methanogenic bacteria. Appl Environ Microbiol. 1983 Mar;45(3):800–803. doi: 10.1128/aem.45.3.800-803.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Murray P. A., Zinder S. H. Nutritional Requirements of Methanosarcina sp. Strain TM-1. Appl Environ Microbiol. 1985 Jul;50(1):49–55. doi: 10.1128/aem.50.1.49-55.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Nolan R. A. Amino acids and growth factors in vitamin-free casamino acids. Mycologia. 1971 Nov-Dec;63(6):1231–1234. [PubMed] [Google Scholar]
  12. Noll K. M., Rinehart K. L., Jr, Tanner R. S., Wolfe R. S. Structure of component B (7-mercaptoheptanoylthreonine phosphate) of the methylcoenzyme M methylreductase system of Methanobacterium thermoautotrophicum. Proc Natl Acad Sci U S A. 1986 Jun;83(12):4238–4242. doi: 10.1073/pnas.83.12.4238. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Paynter M. J., Hungate R. E. Characterization of Methanobacterium mobilis, sp. n., isolated from the bovine rumen. J Bacteriol. 1968 May;95(5):1943–1951. doi: 10.1128/jb.95.5.1943-1951.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Slyter L. L., Weaver J. M. Tetrahydrofolate and other growth requirements of certain strains of Ruminococcus flavefaciens. Appl Environ Microbiol. 1977 Feb;33(2):363–369. doi: 10.1128/aem.33.2.363-369.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. WOLIN E. A., WOLIN M. J., WOLFE R. S. FORMATION OF METHANE BY BACTERIAL EXTRACTS. J Biol Chem. 1963 Aug;238:2882–2886. [PubMed] [Google Scholar]
  16. Whitman W. B., Ankwanda E., Wolfe R. S. Nutrition and carbon metabolism of Methanococcus voltae. J Bacteriol. 1982 Mar;149(3):852–863. doi: 10.1128/jb.149.3.852-863.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Yokoyama M. T., Carlson J. R. Dissimilation of tryptophan and related indolic compounds by ruminal microorganisms in vitro. Appl Microbiol. 1974 Mar;27(3):540–548. doi: 10.1128/am.27.3.540-548.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]

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