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. 1976 Oct;34(4):346–358. doi: 10.1038/bjc.1976.177

Receptors for human immunoglobulin on acute myeloid leukaemic leucocytes.

J C Ridway, G M Taylor, C B Freeman, R Harris
PMCID: PMC2025248  PMID: 823953

Abstract

Immunoglobulin (Fc) receptors were detected on leucocytes from patients with acute myeloid leukaemia (AML) by rosette formation with human cDE/cDE erthyrocytes (HE) sensitized with Rhesus (Rh) antisera (HEA). Of 7 Rh antisera tested, erythrocytes sensitized with anti-d (Gm10) detected the highest numbers of rosette-forming cells (HEA-RFC) in normal and AML leucocyte preparations. Using this assay, HEA-RFC was studied in 22 untreated AML patients and ce assay detected 11-6% lymphocyte and 2-1% granulocyte HEA-RFC in normal peripheral blood. Leucocytes from 16 to 22 AML patients had a similar or lower percentage than normal lymphocyte HEA-RFC, which could be explained by the dilution of peripheral blood leucocytes by poorly or non-rosetting leukaemic blasts. Ten of these 16 patients were diagnosed as having acute myeloblasts leukaemia. Six of the 22 AML patients had high HEA-RFC values of which 5 were diagnosed as having myelomonocytic leukaemia. Cytocentrifuge preparations of HEA-RFC showed that the proportion able to form rosettes was lower in myeloblasts than in monoblasts. Enzyme treatment (pronase), inhibition or simultaneous labelling of surface Ig and Fc receptors showed that the characteristic surface Ig found to AML cells is, at least in part, bound to Fc receptors. The HEA-RFC test described in this paper could be useful in the immuno-diagnosis of myelomonocytic leukaemia.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abramson N., Gelfand E. W., Jandl J. H., Rosen F. S. The interaction between human monocytes and red cells. Specificity for IgG subclasses and IgG fragments. J Exp Med. 1970 Dec 1;132(6):1207–1215. doi: 10.1084/jem.132.6.1207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Abramson N., Lo Buglio A. F., Jandl J. H., Cotran R. S. The interaction between human monocytes and red cells. Binding characteristics. J Exp Med. 1970 Dec 1;132(6):1191–1206. doi: 10.1084/jem.132.6.1191. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brain P., Marston R. H. Rosette formation by human T and B lymphocytes. Eur J Immunol. 1973 Jan;3(1):6–9. doi: 10.1002/eji.1830030103. [DOI] [PubMed] [Google Scholar]
  4. Brown G., Greaves M. F., Lister T. A., Rapson N., Papamichael M. Expression of human T and B lymphocyte cell-surface markers on leukaemic cells. Lancet. 1974 Sep 28;2(7883):753–755. doi: 10.1016/s0140-6736(74)90945-3. [DOI] [PubMed] [Google Scholar]
  5. Dickler H. B., Kunkel H. G. Interaction of aggregated -globulin with B lymphocytes. J Exp Med. 1972 Jul 1;136(1):191–196. doi: 10.1084/jem.136.1.191. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dickler H. B., Sachs D. H. Evidence for identity or close association of the Fc receptor of B lymphocytes and alloantigens determined by the Ir region of the H-2 complex. J Exp Med. 1974 Sep 1;140(3):779–796. doi: 10.1084/jem.140.3.779. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Dickler H. B., Siegal F. P., Bentwich Z. H., Kunkel H. G. Lymphocyte binding of aggregated IgG and surface Ig staining in chronic lymphocytic leukaemia. Clin Exp Immunol. 1973 May;14(1):97–106. [PMC free article] [PubMed] [Google Scholar]
  8. Dickler H. B. Studies of the human lymphocyte receptor for heat-aggregated or antigen-complexed immunoglobulin. J Exp Med. 1974 Aug 1;140(2):508–522. doi: 10.1084/jem.140.2.508. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Froland S. S., Michaelsen T. E., Wisloff F., Natvig J. B. Specificity of receptors for IgG on human lymphocyte-like cells. Scand J Immunol. 1974;3(4):509–517. doi: 10.1111/j.1365-3083.1974.tb01284.x. [DOI] [PubMed] [Google Scholar]
  10. Froland S. S., Natvig J. B. Identification of three different human lymphocyte populations by surface markers. Transplant Rev. 1973;16:114–162. doi: 10.1111/j.1600-065x.1973.tb00119.x. [DOI] [PubMed] [Google Scholar]
  11. Froland S. S., Wisloff F., Michaelsen T. E. Human lymphocytes with receptors for IgG. A population of cells distinct from T- and B-lymphocytes. Int Arch Allergy Appl Immunol. 1974;47(1):124–138. doi: 10.1159/000231207. [DOI] [PubMed] [Google Scholar]
  12. Gutterman J. U., Rossen R. D., Butler W. T., McCredie K. B., Bodey G. P., Sr, Freireich E. J., Hersh E. M. Immunoglobulin on tumor cells and tumor-induced lymphocyte blastogenesis in human acute leukemia. N Engl J Med. 1973 Jan 25;288(4):169–175. doi: 10.1056/NEJM197301252880401. [DOI] [PubMed] [Google Scholar]
  13. Huber H., Douglas S. D., Fudenberg H. H. The IgG receptor: an immunological marker for the characterization of mononuclear cells. Immunology. 1969 Jul;17(1):7–21. [PMC free article] [PubMed] [Google Scholar]
  14. Huber H., Fudenberg H. H. Receptor sites of human monocytes for IgG. Int Arch Allergy Appl Immunol. 1968;34(1):18–31. doi: 10.1159/000230091. [DOI] [PubMed] [Google Scholar]
  15. Messner R. P., Jelinek J. Receptors for human gamma G globulin on human neutrophils. J Clin Invest. 1970 Dec;49(12):2165–2171. doi: 10.1172/JCI106435. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Mårtensson L., Kunkel H. G. Distribution among the gamma-globulin molecules of different genetically determined antigenic specificities in the Gm system. J Exp Med. 1965 Oct 1;122(4):799–811. doi: 10.1084/jem.122.4.799. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Rochna E., Hughes-Jones N. C. The use of purified 125-I-labelled anti-gamma globulin in the determination of the number of D antigen sites on red cells of different phenotypes. Vox Sang. 1965 Nov-Dec;10(6):675–686. doi: 10.1111/j.1423-0410.1965.tb05179.x. [DOI] [PubMed] [Google Scholar]
  18. Wisloff F., Froland S. S., Michaelsen T. E. Antibody-dependent cytotoxicity mediated by human Fc-receptor-bearing cells lacking markers for B- and T-lymphocytes. Int Arch Allergy Appl Immunol. 1974;47(1):139–154. doi: 10.1159/000231208. [DOI] [PubMed] [Google Scholar]

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