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. 1977 Jan;35(1):59–67. doi: 10.1038/bjc.1977.4

Cytotoxicity of guinea-pig lymphoid cells against guinea-pig hepatoma cells in tissue culture.

M Andjargholi, M M Dale
PMCID: PMC2025307  PMID: 188438

Abstract

The cytotoxic effect of guinea-pig lymphoid cells on guinea-pig hepatoma cell lines in tissue culture was investigated, using the microplate technique of Takasugi and Klein (1970). The effect of lymphoid cells from guinea-pigs immunized against tumor cells was compared to that of cells from normal controls. Several ratios of effector to target cells (10 : 1, 50 : 1, 150: 1, 250 : 1) were used. In Hartley guinea-pigs immunized with allogeneic tumour cells, peripheral blood lymphoid cells from 14/16 animals showed significant cytotoxicity against that tumour in culture. In a syngeneic tumour/host system, 7/13 animals showed cytotoxicity. Spleen cells gave less consistent results in both systems. The cytotoxic activity of subpopulations of immune lymphocytes against tumour cells in vitro was investigated. It was found that although both T-cell-enriched and T-cell-depleted cell populations exhibited cytotoxicity against tumour cells, the unfractionated cell population was the most effective. This suggests that some degree of cell cooperation may be involved in the cytotoxicity. Antibody-dependent cellular cytotoxicity was also obtained. A T-cell-depleted population of normal cells was shown to be cytotoxic to tumour cells in the presence of serum from immune animals. This type of cytotoxicity could be obtained concomitantly with cell-mediated cytotoxicity in the same animals.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Basham C., Currie G. A. Development of specific cell-dependent antibody during growth of a syngeneic rat sarcoma. Br J Cancer. 1974 Mar;29(3):189–198. doi: 10.1038/bjc.1974.58. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Berczi I., Sehon A. H. Rejection of tumor cells in vitro: a T-cell-mediated reaction. Int J Cancer. 1975 Oct 15;16(4):665–674. doi: 10.1002/ijc.2910160417. [DOI] [PubMed] [Google Scholar]
  3. Bourne H. R., Lichtenstein L. M., Melmon K. L., Henney C. S., Weinstein Y., Shearer G. M. Modulation of inflammation and immunity by cyclic AMP. Science. 1974 Apr 5;184(4132):19–28. doi: 10.1126/science.184.4132.19. [DOI] [PubMed] [Google Scholar]
  4. Cerottini J. C., Brunner K. T. Cell-mediated cytotoxicity, allograft rejection, and tumor immunity. Adv Immunol. 1974;18:67–132. doi: 10.1016/s0065-2776(08)60308-9. [DOI] [PubMed] [Google Scholar]
  5. Dale M. M., Easty G. C., Tchao R., Desai H., Andjargholi M. The induction of tumours in the guinea-pig with methylcholanthrene and diethylnitrosamine and their propagation in vivo and in vitro. Br J Cancer. 1973 Jun;27(6):445–450. doi: 10.1038/bjc.1973.56. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. FOLEY E. J. Antigenic properties of methylcholanthrene-induced tumors in mice of the strain of origin. Cancer Res. 1953 Dec;13(12):835–837. [PubMed] [Google Scholar]
  7. Greaves M. F., Brown G. Purification of human T and B lymphocytes. J Immunol. 1974 Jan;112(1):420–423. [PubMed] [Google Scholar]
  8. Henney C. S., Bubbers J. E. Studies on the mechanism of lymphocyte-mediated cytolysis. I. The role of divalent cations in cytolysis by T lymphocytes. J Immunol. 1973 Jan;110(1):63–72. [PubMed] [Google Scholar]
  9. Klein G. Experimental studies in tumor imminology. Fed Proc. 1969 Nov-Dec;28(6):1739–1753. [PubMed] [Google Scholar]
  10. Lamon E. W., Wigzell H. In vitro activity of subpopulations of immune lymphocytes against murine sarcoma cells. Transplantation. 1974 Oct;18(4):368–371. doi: 10.1097/00007890-197410000-00012. [DOI] [PubMed] [Google Scholar]
  11. Law L. W. Studies of the significance of tumor antigens in induction and repression of neoplastic diseases: presidential address. Cancer Res. 1969 Jan;29(1):1–21. [PubMed] [Google Scholar]
  12. Leclerc J. C., Gomard E., Plata F., Levy J. P. Cell-mediated immune reaction against tumors induced by oncornaviruses. II. Nature of the effector cells in tumor-cell cytolysis. Int J Cancer. 1973 Mar 15;11(2):426–432. doi: 10.1002/ijc.2910110220. [DOI] [PubMed] [Google Scholar]
  13. Lonai P., Feldman M. Cooperation of lymphoid cells in an in vitro graft reaction system. II. The "bone marrow-derived" cell. Transplantation. 1971 May;11(5):446–456. doi: 10.1097/00007890-197105000-00003. [DOI] [PubMed] [Google Scholar]
  14. MacLennan I. C. Antibody in the induction and inhibition of lymphocyte cytotoxicity. Transplant Rev. 1972;13:67–90. doi: 10.1111/j.1600-065x.1972.tb00060.x. [DOI] [PubMed] [Google Scholar]
  15. Medina D., Heppner G. Cell-mediated "immunostimulation" induced by mammary tumour virus-free Balb-c mammary tumours. Nature. 1973 Mar 30;242(5396):329–330. doi: 10.1038/242329a0. [DOI] [PubMed] [Google Scholar]
  16. Oppenheim J. J., Zbar B., Rapp H. Specific inhibition of tumor cell DNA synthesis in vitro by lymphocytes from peritoneal exudate of immunized syngeneic guinea pigs. Proc Natl Acad Sci U S A. 1970 Aug;66(4):1119–1126. doi: 10.1073/pnas.66.4.1119. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. PREHN R. T., MAIN J. M. Immunity to methylcholanthrene-induced sarcomas. J Natl Cancer Inst. 1957 Jun;18(6):769–778. [PubMed] [Google Scholar]
  18. Perlmann P., Holm G. Cytotoxic effects of lymphoid cells in vitro. Adv Immunol. 1969;11:117–193. doi: 10.1016/s0065-2776(08)60479-4. [DOI] [PubMed] [Google Scholar]
  19. Plata F., Gomard E., Leclerc J. C., Levy J. P. Comparative in vitro studies on effector cell diversity in the cellular immune response to murine sarcoma virus (MSV)-induced tumors in mice. J Immunol. 1974 Apr;112(4):1477–1487. [PubMed] [Google Scholar]
  20. Plaut M., Lichtenstein L. M., Gillespie E., Henney C. S. Studies on the mechanism of lymphocyte-mediated cytolysis. IV. Specificity of the histamine receptor on effector T cells. J Immunol. 1973 Aug;111(2):389–394. [PubMed] [Google Scholar]
  21. Rapp H. J., Churchill W. H., Jr, Kronman B. S., Rolley R. T., Hammond W. G., Borsos T. Antigenicity of a new diethylnitrosamine-induced transplantable guinea pig hepatoma: pathology and formation of ascites variant. J Natl Cancer Inst. 1968 Jul;41(1):1–7. [PubMed] [Google Scholar]
  22. Takasugi M., Klein E. A microassay for cell-mediated immunity. Transplantation. 1970 Mar;9(3):219–227. doi: 10.1097/00007890-197003000-00005. [DOI] [PubMed] [Google Scholar]
  23. Wahl S. M., Iverson G. M., Oppenheim J. J. Induction of guinea pig B-cell lymphokine synthesis by mitogenic and nonmitogenic signals to Fc, Ig, and C3 receptors. J Exp Med. 1974 Dec 1;140(6):1631–1645. doi: 10.1084/jem.140.6.1631. [DOI] [PMC free article] [PubMed] [Google Scholar]

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