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. 1977 Feb;35(2):199–207. doi: 10.1038/bjc.1977.27

Rauscher virus-induced reticulum-cell sarcomas: Their growth in vitro and erythropoietic differentiation.

A H Fieldsteel, P J Dawson, F A Becker, C Kurahara, C Mitoma
PMCID: PMC2025318  PMID: 319819

Abstract

A transplantable reticulum-cell sarcoma induced by Rauscher virus (RV) in (C57BL/6 X DBA/2)F1 (BDF1) mice was grown in tissue culture. Four separate cell lines were established, all of which grew predominantly in suspension. The doubling time of the cells from these cultures ranged from 17 to 32 h. Each culture continued to replicate RV, as indicated by the infectivity in newborn mice of all fluids tested up to the 75th passage. Since the morphological appearance of the cells in vitro was consistent with that of proerythroblasts, all cultures were tested for their ability to differentiate along the erythrocytic line under the influence of dimethylsulphoxide (DMSO). One of the cultures produced small quantities of haemoglobin independently of DMSO. Another was shown to produce haemoglobin, as well as to take up 59Fe and incorporate it into haem, only in the presence of DMSO. The 2 remaining cultures failed to produce haemoglobin, either spontaneously or in the presence of DMSO. Cells from each of the RV-induced cultures, when inoculated back into BDF1 mice, induced typical reticulum-cell sarcomas, without in vivo evidence of erythroid differentiation. In contrast, 2 morphologically identical but non-infectious cell lines derived from Friend virus-induced reticulum-cell sarcomas did not show erythroid differentiation in vivo or in vitro, either in the absence or presence of DMSO.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Braylan R. C., Jaffe E. S., Berard C. W. Malignant lymphomas: current classification and new observations. Pathol Annu. 1975;10:213–270. [PubMed] [Google Scholar]
  2. Brommer E. J., Bentvelzen P. The haematopoietic stem cell in Rauscher virus-induced erythroblastosis of BALB/c mice. Eur J Cancer. 1974 Dec;10(12):827–833. doi: 10.1016/0014-2964(74)90141-8. [DOI] [PubMed] [Google Scholar]
  3. Dawson P. J., Fieldsteel A. H. Reticulum cell sarcomas induced in mice by Rauscher virus. J Natl Cancer Inst. 1974 Jun;52(6):1805–1809. doi: 10.1093/jnci/52.6.1805. [DOI] [PubMed] [Google Scholar]
  4. FIELDSTEEL A. H., DAWSON P. J., BOSTICK W. L. Viral studies on generalized Friend disease and a tumor variant in BALB/c and related hybrid mice. Cancer Res. 1963 Mar;23:355–362. [PubMed] [Google Scholar]
  5. FRIEND C., HADDAD J. R. Tumor formation with transplants of spleen or liver from mice with virus-induced leukemia. J Natl Cancer Inst. 1960 Dec;25:1279–1285. [PubMed] [Google Scholar]
  6. Fieldsteel A. H., Dawson P. J., Kurahara C. In vivo and in vitro recovery of defective Friend virus by various leukemia viruses. Int J Cancer. 1971 Sep 15;8(2):304–309. doi: 10.1002/ijc.2910080216. [DOI] [PubMed] [Google Scholar]
  7. Fieldsteel A. H., Dawson P. J., Scholler J. Friend disease and a tumor variant in hybrid (BDF1) mice. Proc Soc Exp Biol Med. 1968 Feb;127(2):614–617. doi: 10.3181/00379727-127-32755. [DOI] [PubMed] [Google Scholar]
  8. Fieldsteel A. H., Dawson P. J., Scholler J. Virus-free Friend virus-induced tumor: in vitro and in vivo characteristics. J Natl Cancer Inst. 1966 Jan;36(1):71–80. [PubMed] [Google Scholar]
  9. Fieldsteel A. H., Kurahara C. Moloney leukaemia virus as a helper in retrieving Friend virus from a non-infectious reticulum cell sarcoma. Nature. 1969 Sep 20;223(5212):1274–1274. doi: 10.1038/2231274a0. [DOI] [PubMed] [Google Scholar]
  10. Friend C., Scher W., Holland J. G., Sato T. Hemoglobin synthesis in murine virus-induced leukemic cells in vitro: stimulation of erythroid differentiation by dimethyl sulfoxide. Proc Natl Acad Sci U S A. 1971 Feb;68(2):378–382. doi: 10.1073/pnas.68.2.378. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. LOBUE J., DORNFEST B. S., GORDON A. S., HURST J., QUASTLER H. Marrow distribution in rat femurs determined by cell enumeration and Fe59 labeling. Proc Soc Exp Biol Med. 1963 Apr;112:1058–1062. doi: 10.3181/00379727-112-28250. [DOI] [PubMed] [Google Scholar]
  12. Ostertag W., Melderis H., Steinheider G., Kluge N., Dube S. Synthesis of mouse haemoglobin and globin mRNA in leukaemic cell cultures. Nat New Biol. 1972 Oct 25;239(95):231–234. doi: 10.1038/newbio239231a0. [DOI] [PubMed] [Google Scholar]
  13. Pluznik D. H., Sachs L., Resnitzky P. The mechanism of leukemogenesis by the Rauscher leukemia virus. Natl Cancer Inst Monogr. 1966 Sep;22:3–14. [PubMed] [Google Scholar]
  14. Scher W., Holland J. G., Friend C. Hemoglobin synthesis in murine virus-induced leukemic cells in vitro. I. Partial purification and identification of hemoglobins. Blood. 1971 Apr;37(4):428–437. [PubMed] [Google Scholar]
  15. Swetly P., Ostertag W. Friend virus release and induction of haemoglobin synthesis in erythroleukaemic cells respond differently to interferon. Nature. 1974 Oct 18;251(5476):642–644. doi: 10.1038/251642a0. [DOI] [PubMed] [Google Scholar]
  16. Yokoro K., Thorell B. Cytology and pathogenesis of Rauscher virus disease in splenectomized mice. Cancer Res. 1966 Mar;26(3):536–543. [PubMed] [Google Scholar]

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