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. 1977 Jul;36(1):35–40. doi: 10.1038/bjc.1977.151

Non-specific cytotoxicity of spleen cells in mice bearing transplanted chemically induced fibrosarcomas.

A Mantovani, R Evans, P Alexander
PMCID: PMC2025456  PMID: 889683

Abstract

Spleen cells collected from mice bearing transplanted chemically induced syngeneic fibrosarcomas non-specifically inhibited DNA synthesis of sarcoma and lymphoma target cells in vitro. Splenocytes from mice hyper-immunized against a syngeneic sarcoma specifically inhibited DNA synthesis of the tumour used for immunization. The impairment of tumour-cell DNA synthesis was associated in vitro with cytostasis, and lysis of the target cells was not seen. Since treatment with anti-theta serum and complement did not impair cytostatic action of the spleen cells, and since thymus-deprived animals showed similar activity to normal mice, T lymphocytes were not involved in non-specific cytostasis. Removal of phagocytic adherent cells by carbonyl iron markedly inhibited the cytostatic activity of the spleen cells, suggesting a role in this reaction for cells of the monocyte-macrophage series. The presence of an actively growing sarcoma was a prerequisite for the expression of non-specific cytostasis, since surgical excision resulted in complete disappearance of this activity of spleen cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Boyle M. D., Ormerod M. G. The destruction of tumor cells by alloimmune peritoneal cells: mechanism of action of activated macrophages in vitro. J Reticuloendothel Soc. 1975 Feb;17(2):73–83. [PubMed] [Google Scholar]
  2. Currie G. A., Gage J. O. Influence of tumour growth on the evolution of cytotoxic lymphoid cells in rats bearing a spontaneously metastasizing syngeneic fibrosarcoma. Br J Cancer. 1973 Aug;28(2):136–146. doi: 10.1038/bjc.1973.131. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Davies A. J., Carter R. L., Leuchars E., Wallis V. The morphology of immune reactions in normal, thymectomized and reconstituted mice. II. The response to oxazolone. Immunology. 1969 Jul;17(1):111–126. [PMC free article] [PubMed] [Google Scholar]
  4. Evans R., Alexander P. Cooperation of immune lymphoid cells with macrophages in tumour immunity. Nature. 1970 Nov 14;228(5272):620–622. doi: 10.1038/228620a0. [DOI] [PubMed] [Google Scholar]
  5. Evans R., Booth C. G. Inhibition of 125IUdR incorporation by supernatants from macrophage and lymphocyte cultures: a cautionary note. Cell Immunol. 1976 Sep;26(1):120–126. doi: 10.1016/0008-8749(76)90354-3. [DOI] [PubMed] [Google Scholar]
  6. Kirchner H., Holden H. T., Herberman R. B. Inhibition of in vitro growth of lymphoma cells by macrophages from tumor-bearing mice. J Natl Cancer Inst. 1975 Oct;55(4):971–975. doi: 10.1093/jnci/55.4.971. [DOI] [PubMed] [Google Scholar]
  7. Kirchner H., Holden H. T., Herberman Splenic suppressor macrophages induced in mice by injection of Corynebacterium parvum. J Immunol. 1975 Nov;115(5):1212–1216. [PubMed] [Google Scholar]
  8. Lamon E. W., Wigzell H., Andersson B., Klein E. Antitumour activity in vitro dependent on immune B lymphocytes. Nat New Biol. 1973 Aug 15;244(137):209–211. doi: 10.1038/newbio244209a0. [DOI] [PubMed] [Google Scholar]
  9. Lundgren G., Zukoski C. F., Möller G. Differential effects of human granulocytes and lymphocytes on human fibroblasts in vitro. Clin Exp Immunol. 1968 Oct;3(8):817–836. [PMC free article] [PubMed] [Google Scholar]
  10. Meerpohl H. G., Lohmann-Matthes M. L., Fischer H. Studies on the activation of mouse bone marrow-derived macrophages by the macrophage cytotoxicity factor (MCF). Eur J Immunol. 1976 Mar;6(3):213–217. doi: 10.1002/eji.1830060313. [DOI] [PubMed] [Google Scholar]
  11. Plata F., Gomard E., Leclerc J. C., Levy J. P. Further evidence for the involvement of thymus-processed lymphocytes in syngeneic tumor cell cytolysis. J Immunol. 1973 Sep;111(3):667–671. [PubMed] [Google Scholar]
  12. REIF A. E., ALLEN J. M. THE AKR THYMIC ANTIGEN AND ITS DISTRIBUTION IN LEUKEMIAS AND NERVOUS TISSUES. J Exp Med. 1964 Sep 1;120:413–433. doi: 10.1084/jem.120.3.413. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Seeger R. C., Rayner S. A., Owen J. J. An analysis of variables affecting the measurement of tumor immunity in vitro with 125I-iododeoxyuridine-labelled target cells. Studies of immunity to primary Moloney sarcomas. Int J Cancer. 1974 May 15;13(5):697–713. doi: 10.1002/ijc.2910130514. [DOI] [PubMed] [Google Scholar]
  14. Thomson D. M., Sellens V., Eccles S., Alexander P. Radioimmunoassay of tumour specific transplantation antigen of a chemically induced rat sarcoma: circulating soluble tumour antigen in tumour bearers. Br J Cancer. 1973 Nov;28(5):377–388. doi: 10.1038/bjc.1973.163. [DOI] [PMC free article] [PubMed] [Google Scholar]

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