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. 1977 Aug;36(2):215–220. doi: 10.1038/bjc.1977.180

Cell kinetics of urethane-induced murine pulmonary adenomas: III. Implications of the disparity between the rates of entry into DNA synthesis and into mitosis.

P Dyson, A G Heppleston
PMCID: PMC2025475  PMID: 911659

Abstract

Metaphase arrest by vincristine in urethane-induced murine pulmonary adenomas became linear after an interval of 60 min. The rate of entry into metaphase was 0-191%/h, which was considerably less than the 1%/h for the rate of entry into DNA synthesis obtained previously by double labelling. The duration of prophase plus metaphase was calculated to be 1-7 h. A growth fraction of 9% and a cell-loss factor of 52% were derived. The disparity between rates of entry into DNA synthesis and into metaphase was investigated by microdensitometry on Feulgen-stained squash preparations of tumours of varying ages. Tne DNA profiles showed an increasing frequency of hyperdiploid nuclei with age. Circumstantial evidence for polyploidy was provided by the presence of many binucleate cells in the tumours. By analogy with the liver, these cells may well represent a stage in the development of polyploidy, and the possible relevance of these findings to the neoplastic process is considered.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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