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. 1977 Dec;36(6):713–722. doi: 10.1038/bjc.1977.254

In vitro immune responses to PPD, extracts from Raji cells and nasopharyngeal carcinoma biopsies in NPC leucocytes.

W S Ng, M H Ng, H C Ho, J P Lamelin
PMCID: PMC2025569  PMID: 597472

Abstract

Peripheral leucocytes from nasopharyngeal carcinoma (NPC) patients and control subjects, which included healthy subjects and patients with other cancers, have been tested against PPD and a panel of extracts from Raji cells and pooled NPC biopsies, using the blast transformation and the macrophage migration inhibition assays. The results of both assays indicated that the in vitro cell-mediated immune (CMI) responses to the Raji-cell extracts and NPC-biopsy extracts were associated with NPC. However, the peripheral leucocytes from NPC patients and control subjects responded similarly to PPD. These results are in general accord with the skin tests reported by Levine et al. (1976) and Ho, Ng and Kwan (1977b). The antigenic specificity of the NPC-associated CMI responses remains, however, to be resolved, as the extracts used in these or in the in vivo CMI studies were heterogeneous mixtures.

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Selected References

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  1. Chan S. H., Chew T. S., Goh E. H., Simons M. J., Shanmugaratnam K. Impaired general cell-mediated immune functions in vivo and in vitro in patients with nasopharyngeal carcinoma. Int J Cancer. 1976 Aug 15;18(2):139–144. doi: 10.1002/ijc.2910180202. [DOI] [PubMed] [Google Scholar]
  2. Derge J. G., Martos L. M., Tagamets M. A., Chang S. Y., Chakrabarty M. Identification of a critical period during the S phase for activation of the Epstein-Barr virus by 5-iododeoxyuridine. Nat New Biol. 1973 Aug 15;244(137):214–217. doi: 10.1038/newbio244214a0. [DOI] [PubMed] [Google Scholar]
  3. Desgranges C., Wolf H., De-Thé G., Shanmugaratnam K., Cammoun N., Ellouz R., Klein G., Lennert K., Muñoz N., Zur Hausen H. Nasopharyngeal carcinoma. X. Presence of epstein-barr genomes in separated epithelial cells of tumours in patients from Singapore, Tunisia and Kenya. Int J Cancer. 1975 Jul 15;16(1):7–15. doi: 10.1002/ijc.2910160103. [DOI] [PubMed] [Google Scholar]
  4. Eife R. F., Eife G., August C. S., Kuhre W. L., Staehr-Johansen K. Lymphotoxin production and blast cell transformation by cord blood lymphocytes: dissociated lymphocyte function in newborn infants. Cell Immunol. 1974 Dec;14(3):435–442. doi: 10.1016/0008-8749(74)90194-4. [DOI] [PubMed] [Google Scholar]
  5. Glaser R., de Thé G., Lenoir G., Ho J. H. Superinfection epithelial nasopharyngeal carcinoma cells with Epstein-Barr virus. Proc Natl Acad Sci U S A. 1976 Mar;73(3):960–963. doi: 10.1073/pnas.73.3.960. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Habel K. Antigens of virus-induced tumors. Adv Immunol. 1969;10:229–250. doi: 10.1016/s0065-2776(08)60418-6. [DOI] [PubMed] [Google Scholar]
  7. Henle G., Henle W., Klein G. Demonstration of two distinct components in the early antigen complex of Epstein-Barr virus-infected cells. Int J Cancer. 1971 Sep 15;8(2):272–282. doi: 10.1002/ijc.2910080212. [DOI] [PubMed] [Google Scholar]
  8. Henle W., Henle G., Ho H. C., Burtin P., Cachin Y., Clifford P., de Schryver A., de-Thé G., Diehl V., Klein G. Antibodies to Epstein-Barr virus in nasopharyngeal carcinoma, other head and neck neoplasms, and control groups. J Natl Cancer Inst. 1970 Jan;44(1):225–231. [PubMed] [Google Scholar]
  9. Henle W., Ho H. C., Henle G., Kwan H. C. Antibodies to Epstein-Barr virus-related antigens in nasopharyngeal carcinoma. Comparison of active cases with long-term survivors. J Natl Cancer Inst. 1973 Aug;51(2):361–369. [PubMed] [Google Scholar]
  10. Ho H. C., Ng M. H., Kwan H. C., Chau J. C. Epstein-Barr-virus-specific IgA and IgG serum antibodies in nasopharyngeal carcinoma. Br J Cancer. 1976 Dec;34(6):655–660. doi: 10.1038/bjc.1976.228. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Ho H. C., Ng M. H., Kwan H. C. IgA antibodies to Epstein-Barr viral capsid antigens in saliva of nasopharyngeal carcinoma patients. Br J Cancer. 1977 Jun;35(6):888–890. doi: 10.1038/bjc.1977.134. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Huang D. P., Ho J. H., Henle W., Henle G. Demonstration of Epstein-Barr virus-associated nuclear antigen in nasopharyngeal carcinoma cells from fresh biopsies. Int J Cancer. 1974 Nov 15;14(5):580–588. doi: 10.1002/ijc.2910140504. [DOI] [PubMed] [Google Scholar]
  13. Klein G., Giovanella B. C., Lindahl T., Fialkow P. J., Singh S., Stehlin J. S. Direct evidence for the presence of Epstein-Barr virus DNA and nuclear antigen in malignant epithelial cells from patients with poorly differentiated carcinoma of the nasopharynx. Proc Natl Acad Sci U S A. 1974 Dec;71(12):4737–4741. doi: 10.1073/pnas.71.12.4737. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Lai P. K., Alpers M. P., Mackay Scollay E. M. In vitro evaluation of cell-mediated immunity to Epstein-Barr herpesvirus by cell migration inhibition tests. J Natl Cancer Inst. 1975 Dec;55(6):1319–1322. doi: 10.1093/jnci/55.6.1319. [DOI] [PubMed] [Google Scholar]
  15. Levine P. H., De Thé G. B., Brugere G., Schwaab G., Mourali N., Herberman R. B., Ambrosioni J. C., Revol P. Immunity to antigens associated with a cell line derived from nasopharyngeal cancer (NPC) in non-Chinese NPC patients. Int J Cancer. 1976 Feb 15;17(2):155–160. doi: 10.1002/ijc.2910170202. [DOI] [PubMed] [Google Scholar]
  16. Lo E. H., Ng M. H., Ng W. S., Lui S., Ho H. C. Study on the surface polypeptides isolated from human lymphoblastoid ce-ls by ionic shock. Biochim Biophys Acta. 1976 Oct 22;444(3):863–874. doi: 10.1016/0304-4165(76)90332-9. [DOI] [PubMed] [Google Scholar]
  17. Nkrumah F., Henle W., Henle G., Herberman R., Perkins V., Depue R. Burkitt's lymphoma: its clinical course in relation to immunologic reactivities to Epstein-Barr virus and tumor-related antigens. J Natl Cancer Inst. 1976 Nov;57(5):1051–1056. doi: 10.1093/jnci/57.5.1051. [DOI] [PubMed] [Google Scholar]
  18. Reedman B. M., Klein G. Cellular localization of an Epstein-Barr virus (EBV)-associated complement-fixing antigen in producer and non-producer lymphoblastoid cell lines. Int J Cancer. 1973 May;11(3):499–520. doi: 10.1002/ijc.2910110302. [DOI] [PubMed] [Google Scholar]
  19. Rocklin R. E., Meyers O. L., David J. R. An in vitro assay for cellular hypersensitivity in man. J Immunol. 1970 Jan;104(1):95–102. [PubMed] [Google Scholar]
  20. Svedmyr E., Jondal M. Cytotoxic effector cells specific for B Cell lines transformed by Epstein-Barr virus are present in patients with infectious mononucleosis. Proc Natl Acad Sci U S A. 1975 Apr;72(4):1622–1626. doi: 10.1073/pnas.72.4.1622. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Trumper P. A., Epstein M. A., Giovanella B. C. Activation in vitro by BUdR of a productive EB virus infection in the epithelial cells of nasopharyngeal carcinoma. Int J Cancer. 1976 May 15;17(5):578–587. doi: 10.1002/ijc.2910170505. [DOI] [PubMed] [Google Scholar]
  22. Valdimarsson H., Wood C. B., Hobbs J. R., Holt P. J. Immunological features in a case of chronic granulomatous candidiasis and its treatment with transfer factor. Clin Exp Immunol. 1972 Jun;11(2):151–163. [PMC free article] [PubMed] [Google Scholar]
  23. Wolf H., zur Hausen H., Becker V. EB viral genomes in epithelial nasopharyngeal carcinoma cells. Nat New Biol. 1973 Aug 22;244(138):245–247. doi: 10.1038/newbio244245a0. [DOI] [PubMed] [Google Scholar]
  24. de Schryver A., Klein G., Henle W., Henle G. EB virus-associated antibodies in Caucasian patients with carcinoma of the nasopharynx and in long-term survivors after treatment. Int J Cancer. 1974 Mar 15;13(3):319–325. doi: 10.1002/ijc.2910130307. [DOI] [PubMed] [Google Scholar]
  25. de-Thé G., Ho J. H., Ablashi D. V., Day N. E., Macario A. J., Martin-Berthelon M. C., Pearson G., Sohier R. Nasopharyngeal carcinoma. IX. Antibodies to EBNA and correlation with response to other ebv antigens in chinese patients. Int J Cancer. 1975 Nov 15;16(5):713–721. doi: 10.1002/ijc.2910160503. [DOI] [PubMed] [Google Scholar]

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