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. 1988 Apr;54(4):851–854. doi: 10.1128/aem.54.4.851-854.1988

New enrichment method for isolation of pathogenic Yersinia enterocolitica serogroup O:3 from pork.

G Wauters 1, V Goossens 1, M Janssens 1, J Vandepitte 1
PMCID: PMC202562  PMID: 3377499

Abstract

A new enrichment medium for the recovery of pathogenic Yersinia enterocolitica serogroup O:3 from naturally infected meat products based on three selective agents, Irgasan, ticarcillin, and potassium chlorate (ITC), was compared with several other one- or two-step enrichments. Y. enterocolitica serogroup O:3 was recovered from 96.5% of 29 pork tongues, 24% of 50 ground pork samples, 16% of 25 masseter muscle samples, and 61% of tonsils. ITC was by far the most sensitive method for the recovery of Y. enterocolitica O:3, especially from ground meat and masseter muscles, while cold and two-step enrichments yielded better results for nonpathogenic strains. Plating of ITC enrichments onto SS-deoxycholate-calcium agar gave overall better results than plating onto cefsulodin-Irgasan-novobiocin agar for serogroup O:3.

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Selected References

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