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. 1988 May;54(5):1099–1103. doi: 10.1128/aem.54.5.1099-1103.1988

Anti-Candida albicans activity of Pichia anomala as determined by a growth rate reduction assay.

A D Sawant 1, A T Abdelal 1, D G Ahearn 1
PMCID: PMC202610  PMID: 3291760

Abstract

Killer toxin activity of Pichia anomala WC65 appeared fungicidal for P. bimundalis WC38 and fungistatic for Candida albicans RC1. Inhibitory activity against sensitive C. albicans showed a linear relationship between toxin concentrations and the inverse of the reduced growth rates. The plot of toxin concentrations against growth rates was hyperbolic, as is characteristic of saturation kinetics. Sensitivity of C. albicans to the toxin decreased with increased cell age. The measurement of growth rate reduction provided a simple and accurate method for quantitation of toxin.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Al-Aidroos K., Bussey H. Chromosomal mutants of Saccharomyces cerevisiae affecting the cell wall binding site for killer factor. Can J Microbiol. 1978 Mar;24(3):228–237. doi: 10.1139/m78-041. [DOI] [PubMed] [Google Scholar]
  2. Berry E. A., Bevan E. A. A new species of double-stranded RNA from yeast. Nature. 1972 Sep 29;239(5370):279–280. doi: 10.1038/239279a0. [DOI] [PubMed] [Google Scholar]
  3. Bussey H. Physiology of killer factor in yeast. Adv Microb Physiol. 1981;22:93–122. doi: 10.1016/s0065-2911(08)60326-4. [DOI] [PubMed] [Google Scholar]
  4. Bussey H., Sherman D., Somers J. M. Action of yeast killer factor: a resistant mutant with sensitive spheroplasts. J Bacteriol. 1973 Mar;113(3):1193–1197. doi: 10.1128/jb.113.3.1193-1197.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bussey H., Skipper N. Membrane-mediated killing of Saccharomyces cerevisiae by glycoproteins from Torulopsis glabrata. J Bacteriol. 1975 Oct;124(1):476–483. doi: 10.1128/jb.124.1.476-483.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gunge N., Tamaru A., Ozawa F., Sakaguchi K. Isolation and characterization of linear deoxyribonucleic acid plasmids from Kluyveromyces lactis and the plasmid-associated killer character. J Bacteriol. 1981 Jan;145(1):382–390. doi: 10.1128/jb.145.1.382-390.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hutchins K., Bussey H. Cell wall receptor for yeast killer toxin: involvement of (1 leads to 6)-beta-D-glucan. J Bacteriol. 1983 Apr;154(1):161–169. doi: 10.1128/jb.154.1.161-169.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kurtzman C. P. Synonomy of the yeast genera Hansenula and Pichia demonstrated through comparisons of deoxyribonucleic acid relatedness. Antonie Van Leeuwenhoek. 1984;50(3):209–217. doi: 10.1007/BF02342132. [DOI] [PubMed] [Google Scholar]
  9. Mahler H. R., Lin C. C. Exogenous adenosine 3': 5'-monophosphate can release yeast from catabolite repression. Biochem Biophys Res Commun. 1978 Aug 14;83(3):1039–1047. doi: 10.1016/0006-291x(78)91500-0. [DOI] [PubMed] [Google Scholar]
  10. Middelbeek E. J., Hermans J. M., Stumm C. Production, purification and properties of a Pichia kluyveri killer toxin. Antonie Van Leeuwenhoek. 1979;45(3):437–450. doi: 10.1007/BF00443282. [DOI] [PubMed] [Google Scholar]
  11. Morace G., Archibusacci C., Sestito M., Polonelli L. Strain differentiation of pathogenic yeasts by the killer system. Mycopathologia. 1984 Feb 15;84(2-3):81–85. doi: 10.1007/BF00436517. [DOI] [PubMed] [Google Scholar]
  12. Polonelli L., Archibusacci C., Sestito M., Morace G. Killer system: a simple method for differentiating Candida albicans strains. J Clin Microbiol. 1983 May;17(5):774–780. doi: 10.1128/jcm.17.5.774-780.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Sugisaki Y., Gunge N., Sakaguchi K., Yamasaki M., Tamura G. Characterization of a novel killer toxin encoded by a double-stranded linear DNA plasmid of Kluyveromyces lactis. Eur J Biochem. 1984 Jun 1;141(2):241–245. doi: 10.1111/j.1432-1033.1984.tb08183.x. [DOI] [PubMed] [Google Scholar]
  14. Sugisaki Y., Gunge N., Sakaguchi K., Yamasaki M., Tamura G. Kluyveromyces lactis killer toxin inhibits adenylate cyclase of sensitive yeast cells. Nature. 1983 Aug 4;304(5925):464–466. doi: 10.1038/304464a0. [DOI] [PubMed] [Google Scholar]
  15. Tipper D. J., Bostian K. A. Double-stranded ribonucleic acid killer systems in yeasts. Microbiol Rev. 1984 Jun;48(2):125–156. doi: 10.1128/mr.48.2.125-156.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Wickner R. B. Killer systems in Saccharomyces cerevisiae: three distinct modes of exclusion of M2 double-stranded RNA by three species of double-stranded RNA, M1, L-A-E, and L-A-HN. Mol Cell Biol. 1983 Apr;3(4):654–661. doi: 10.1128/mcb.3.4.654. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Woods D. R., Bevan E. A. Studies on the nature of the killer factor produced by Saccharomyces cerevisiae. J Gen Microbiol. 1968 Apr;51(1):115–126. doi: 10.1099/00221287-51-1-115. [DOI] [PubMed] [Google Scholar]
  18. Young T. W., Yagiu M. A comparison of the killer character in different yeasts and its classification. Antonie Van Leeuwenhoek. 1978;44(1):59–77. doi: 10.1007/BF00400077. [DOI] [PubMed] [Google Scholar]
  19. de la Peña P., Barros F., Gascón S., Ramos S., Lazo P. S. Primary effects of yeast killer toxin. Biochem Biophys Res Commun. 1980 Sep 30;96(2):544–550. doi: 10.1016/0006-291x(80)91390-x. [DOI] [PubMed] [Google Scholar]

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